RRC ID |
17455
|
著者 |
Hollmann A, Delfederico L, De Antoni G, Semorile L, Disalvo EA.
|
タイトル |
Interaction of bacterial surface layer proteins with lipid membranes: synergysm between surface charge density and chain packing.
|
ジャーナル |
Colloids Surf B Biointerfaces
|
Abstract |
S-layer proteins from Lactobacillus kefir and Lactobacillus brevis are able to adsorb on the surface of positively charged liposomes composed by Soybean lecithin, cholesterol and stearylamine. The different K values for S-layer proteins isolated from L. kefir and L. brevis (4.22 x 10(-3) and 2.45 x 10(2) microM(-1) respectively) indicates that the affinity of the glycosylated protein isolated from L. kefir is higher than the non-glycosylated one. The attachment of S-layer proteins counteracts the electrostatic charge repulsion between stearylamine molecules in the membrane surface, producing an increase in the rigidity in the acyl chains as measured by DPH anisotropy. Laurdan generalized polarization (GP) shows that glycosylated causes a GP increase, attributed to a lowering in water penetration into the head groups of membrane phospholipids, with charge density reduction, while the non-glycosylated does not affect it. The octadecyl-rhodamine results indicate that S-layer coated liposomes do not show spontaneous dequenching in comparison with control liposomes without S-layer proteins, suggesting that S-layer protein avoid spontaneous liposomal fusion. It is concluded that the increase in stability of liposomes coated with S-layers proteins is due to the higher rigidity induced by the S-layer attachment by electrostatic forces.
|
巻・号 |
79(1)
|
ページ |
191-7
|
公開日 |
2010-8-1
|
DOI |
10.1016/j.colsurfb.2010.03.046
|
PII |
S0927-7765(10)00194-3
|
PMID |
20417069
|
MeSH |
Algorithms
Bacterial Proteins / chemistry*
Freeze Fracturing
Lactobacillus / chemistry
Lactobacillus brevis / chemistry
Liposomes / chemistry*
Liposomes / ultrastructure
Membrane Glycoproteins / chemistry*
Membrane Lipids / chemistry*
Microscopy, Electron, Transmission
Models, Chemical
Models, Molecular
Species Specificity
|
IF |
4.389
|
引用数 |
11
|
WOS 分野
|
BIOPHYSICS
CHEMISTRY, PHYSICAL
MATERIALS SCIENCE, BIOMATERIALS
|
リソース情報 |
一般微生物 |
JCM 1059
JCM 5818 |