論文 - 詳細
| RRC ID | 17574 |
|---|---|
| 著者 | Ooka H, Kanda S, Okazaki H, Suzuki H, Mishima K, Saito I, Yagi M, Tomoda K, Nishiyama T. |
| タイトル | Characterization of side population (SP) cells in murine cochlear nucleus. |
| ジャーナル | Acta Otolaryngol |
| Abstract |
CONCLUSION:We characterized side population (SP) cells in the cochlear nucleus (CN). Some genes of stem/progenitor markers in sorted SP cells were identified by microarray analysis and RT-PCR. Furthermore, some cells in the CN also demonstrated self-renewal and clonal expansion activities. These results suggest that tissue stem/ progenitor like cells would be identified and characterized as a slow cycling and immaturity in SP cells of CN. OBJECTIVES:SP cells were sorted and characterized as regards their activity in the CN in order to identify the tissue progenitor/stem cells in the auditory nervous system. METHODS:Bromodeoxyuridine (BrdU)-injected mice were prepared and the long-term BrdU-retaining cells were detected by flow cytometry. Gene expression of SP and MP cells was analyzed by microarray analysis and RT-PCR. SP cells were cultured in conditioned medium to expand stem/progenitor cells in vitro and to estimate the spheroid-forming activity of stem cells. RESULTS:In all, 1% of cells in the CN were detected as BrdU-positive. SP cells were detected at a frequency of 4.4% and expressed stem/progenitor markers, Abcb1b, Abcg2, Sca1, Notch1, Notch4, Hes1, and Jag1 in microarray analysis. Expression of Abcb1b, Abcg2, Sca1,Oct3/4, and Sox2 as determined by RT-PCR was supported by the microarray data. CN cells also had sphere-forming activity in young mice, but this activity was decreased by aging. |
| 巻・号 | 132(7) |
| ページ | 693-701 |
| 公開日 | 2012-7-1 |
| DOI | 10.3109/00016489.2012.657358 |
| PMID | 22667338 |
| MeSH | Animals Auditory Pathways / cytology* Auditory Pathways / metabolism Biomarkers / metabolism Cell Cycle / physiology Cochlear Nucleus / cytology* Cochlear Nucleus / metabolism Mice Mice, Inbred C57BL Side-Population Cells / cytology Side-Population Cells / physiology* Transcription Factors / genetics Transcription Factors / metabolism Up-Regulation / physiology |
| IF | 1.157 |
| 引用数 | 6 |
| WOS 分野 | OTORHINOLARYNGOLOGY |
| リソース情報 | |
| 実験動物マウス | RBRC00267 |