| RRC ID |
17677
|
| Author |
Tamaki K, Otaka M, Shibuya T, Sakamoto N, Yamamoto S, Odashima M, Itoh H, Watanabe S.
|
| Title |
Traditional Herbal Medicine, Rikkunshito, Induces HSP60 and Enhances Cytoprotection of Small Intestinal Mucosal Cells as a Nontoxic Chaperone Inducer.
|
| Journal |
Evid Based Complement Alternat Med
|
| Abstract |
Increasing incidence of small intestinal ulcers associated with nonsteroidal anti-inflammatory drugs (NSAIDs) has become a topic with recent advances of endoscopic technology. However, the pathogenesis and therapy are not fully understood. The aim of this study is to examine the effect of Rikkunshito (TJ-43), a traditional herbal medicine, on expression of HSP60 and cytoprotective ability in small intestinal cell line (IEC-6). Effect of TJ-43 on HSP60 expression in IEC-6 cells was evaluated by immunoblot analysis. The effect of TJ-43 on cytoprotective abilities of IEC-6 cells against hydrogen peroxide or indomethacin was studied by MTT assay, LDH-release assay, caspase-8 activity, and TUNEL. HSP60 was significantly induced by TJ-43. Cell necrosis and apoptosis were significantly suppressed in IEC-6 cells pretreated by TJ-43 with overexpression of HSP60. Our results suggested that HSP60 induced by TJ-43 might play an important role in protecting small intestinal epithelial cells from apoptosis and necrosis in vitro.
|
| Volume |
2012
|
| Pages |
278958
|
| Published |
2012-1-1
|
| DOI |
10.1155/2012/278958
|
| PMID |
21822440
|
| PMC |
PMC3140780
|
| IF |
1.813
|
| Times Cited |
6
|
| Resource |
| Human and Animal Cells |
IEC 6(RCB0993) |
