| RRC ID |
1797
|
| 著者 |
Chertemps T, Duportets L, Labeur C, Ueda R, Takahashi K, Saigo K, Wicker-Thomas C.
|
| タイトル |
A female-biased expressed elongase involved in long-chain hydrocarbon biosynthesis and courtship behavior in Drosophila melanogaster.
|
| ジャーナル |
Proc Natl Acad Sci U S A
|
| Abstract |
Drosophila melanogaster produces sexually dimorphic cuticular pheromones that are a key component of the courtship behavior leading to copulation. These molecules are hydrocarbons, with lengths of 23 and 25 carbons in males (mainly with one double bond) and 27 and 29 carbons in females (mainly with two double bonds). Here, we describe an elongase gene, eloF, with female-biased expression. The 771-bp ORF encodes a 257-aa protein that shows the highest sequence identity with mouse SSC1 elongase (33%). The activity of the cDNA expressed in yeast was elongation of saturated and unsaturated fatty acids up to C30. RNAi knockdown in Drosophila led to a dramatic modification of female hydrocarbons, with decreased C29 dienes and increased C25 dienes accompanied by a modification of several courtship parameters: an increase in copulation latency and a decrease in both copulation attempts and copulation. Feminization of the hydrocarbon profile in males by using targeted expression of the transformer gene resulted in high expression levels of eloF, suggesting that the gene is under the control of the sex-determination hierarchy. There is no expression of eloF in Drosophila simulans, which synthesize only C23 and C25 hydrocarbons. These results strongly support the hypothesis that eloF is a crucial enzyme for female pheromone biosynthesis and courtship behavior in D. melanogaster.
|
| 巻・号 |
104(11)
|
| ページ |
4273-8
|
| 公開日 |
2007-3-13
|
| DOI |
10.1073/pnas.0608142104
|
| PII |
0608142104
|
| PMID |
17360514
|
| PMC |
PMC1838592
|
| MeSH |
Acetyltransferases / genetics
Acetyltransferases / physiology*
Amino Acid Sequence
Animals
Drosophila / genetics
Drosophila melanogaster
Female
Hydrocarbons / chemistry
Hydrocarbons / metabolism*
Male
Molecular Sequence Data
Pheromones / metabolism
Saccharomyces cerevisiae / metabolism
Sequence Homology, Amino Acid
Sex Attractants / genetics
Sex Factors
Sexual Behavior, Animal
|
| IF |
9.412
|
| 引用数 |
82
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
16905R-1
16905R-9 |
