論文 - 詳細
| RRC ID | 18117 |
|---|---|
| 著者 | Ochiai K, Yoshikawa Y, Yoshimatsu K, Oonuma T, Tomioka Y, Takeda E, Arikawa J, Mominoki K, Omi T, Hashizume K, Morimatsu M. |
| タイトル | Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51. |
| ジャーナル | FEBS Lett |
| Abstract |
The breast cancer susceptibility protein BRCA2 is essential for recombinational DNA repair. BRCA2 specifically binds to RAD51 via eight BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks. In this study, a mammalian two-hybrid assay and competitive ELISA showed that the interaction between BRC repeat 4 (BRC4) and RAD51 was strengthened by the substitution of a single BRC4 amino acid from valine to isoleucine (V1532I). However, the cancer-associated V1532F mutant exhibited very weak interaction with RAD51. This study used a comparative analysis of BRC4 between animal species to identify V1532 as an important residue that interacts with RAD51. |
| 巻・号 | 585(12) |
| ページ | 1771-7 |
| 公開日 | 2011-6-23 |
| DOI | 10.1016/j.febslet.2011.05.027 |
| PII | S0014-5793(11)00391-7 |
| PMID | 21601571 |
| MeSH | Amino Acid Substitution Animals BRCA2 Protein / metabolism* Humans Mutant Proteins Neoplasm Proteins Protein Binding / genetics Rad51 Recombinase / metabolism* Repetitive Sequences, Nucleic Acid* Two-Hybrid System Techniques Valine / metabolism* |
| IF | 3.057 |
| 引用数 | 15 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |