| RRC ID |
18161
|
| 著者 |
Odkhuu E, Koide N, Haque A, Tsolmongyn B, Naiki Y, Hashimoto S, Komatsu T, Yoshida T, Yokochi T.
|
| タイトル |
Inhibition of receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast formation by pyrroloquinoline quinine (PQQ).
|
| ジャーナル |
Immunol Lett
|
| Abstract |
The effect of pyrroloquinoline quinine (PQQ) on receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast formation was examined using RAW 264.7 macrophage-like cells. RANKL led to the formation of osteoclasts identified as tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells in the culture of RAW 264.7 cells. However, PQQ inhibited the appearance of osteoclasts and prevented the decrease of F4/80 macrophage maturation marker on RANKL-stimulated cells, suggesting a preventive action of PQQ on RANKL-induced osteoclast differentiation. PQQ inhibited the activation of nuclear factor of activated T cells (NFATc1), a key transcription factor of osteoclastogenesis, in RANKL-stimulated cells. On the other hand, PQQ did not inhibit the signaling pathway from RANK/RANKL binding to NFATc1 activation, including NF-κB and mitogen-activated protein kinases (MAPKs). PQQ augmented the expression of type I interferon receptor (IFNAR) and enhanced the IFN-β-mediated janus kinase (JAK1) and signal transducer and activator of transcription (STAT1) expression. Moreover, PQQ reduced the expression level of c-Fos leading to the activation of NFATc1. Taken together, PQQ was suggested to prevent RANKL-induced osteoclast formation via the inactivation of NFATc1 by reduced c-Fos expression. The reduced c-Fos expression might be mediated by the enhanced IFN-β signaling due to augmented IFNAR expression.
|
| 巻・号 |
142(1-2)
|
| ページ |
34-40
|
| 公開日 |
2012-2-29
|
| DOI |
10.1016/j.imlet.2011.12.001
|
| PII |
S0165-2478(11)00259-8
|
| PMID |
22193059
|
| MeSH |
Animals
Cell Differentiation / drug effects*
Cell Line
Interferon-beta / metabolism
Macrophages / cytology
Macrophages / drug effects*
Macrophages / metabolism
Mice
NFATC Transcription Factors / antagonists & inhibitors*
NFATC Transcription Factors / metabolism
Osteoclasts / cytology*
Osteoclasts / drug effects*
Osteoclasts / metabolism
PQQ Cofactor / pharmacology*
Proto-Oncogene Proteins c-fos / metabolism
RANK Ligand / metabolism
RANK Ligand / pharmacology*
Receptor Activator of Nuclear Factor-kappa B / metabolism
Receptor, Interferon alpha-beta / metabolism
Signal Transduction
|
| IF |
3.276
|
| 引用数 |
10
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
