論文 - 詳細
| RRC ID | 18164 |
|---|---|
| 著者 | Furukawa R, Yamada Y, Takenaga M, Igarashi R, Harashima H. |
| タイトル | Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The anti-oxidant enzyme superoxide dismutase (SOD) has the potential for use as a therapeutic agent in the treatment of various diseases caused by reactive oxygen species. However, achieving this would be difficult without a suitable delivery system for SOD. We previously reported that PC-SOD, in which four molecules of a phosphatidylcholine (PC) derivative were covalently bound to each dimer of recombinant human CuZnSOD, was a high affinity for the cell membrane [14]. Here, we show that an octaarginine (R8) modified liposome equipped with PC-SOD (R8-LP (PC-SOD)) enhances its anti-oxidant effect. High-density R8-modified liposomes can stimulate macropinocytosis and are taken up efficiently by cells as demonstrated in a previous study [21]. Flow cytometry analyses showed that R8-LP (PC-SOD) was taken up by cells more efficiently than PC-SOD. Moreover, R8-LP (PC-SOD) liposomes were found to scavenge superoxide anions (O(2)(-)) very efficiently. These results suggest that the efficient cytosolic delivery of PC-SOD by R8-modified liposomes would enhance the anti-oxidant effects of PC-SOD. |
| 巻・号 | 404(3) |
| ページ | 796-801 |
| 公開日 | 2011-1-21 |
| DOI | 10.1016/j.bbrc.2010.12.062 |
| PII | S0006-291X(10)02300-4 |
| PMID | 21168389 |
| MeSH | Antioxidants / administration & dosage* Antioxidants / chemistry Antioxidants / metabolism* Flow Cytometry HeLa Cells Humans Liposomes Oligopeptides / chemistry* Phosphatidylcholines / administration & dosage* Phosphatidylcholines / chemistry Phosphatidylcholines / metabolism* Superoxide Dismutase / administration & dosage* Superoxide Dismutase / chemistry Superoxide Dismutase / metabolism* |
| IF | 2.985 |
| 引用数 | 20 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |