| RRC ID |
18202
|
| 著者 |
Iizuka S, Kudo Y, Yoshida M, Tsunematsu T, Yoshiko Y, Uchida T, Ogawa I, Miyauchi M, Takata T.
|
| タイトル |
Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via cross talk between integrin beta1 and CD63.
|
| ジャーナル |
Mol Cell Biol
|
| Abstract |
Ameloblastin, the most abundant nonamelogenin enamel matrix protein, plays a role in ameloblast differentiation. Here, we found that ameloblastin was expressed in osteosarcoma cells; to explore the potential functions of ameloblastin in osteoblasts, we investigated whether this protein is involved in osteogenic differentiation and bone formation on the premise that CD63, a member of the transmembrane-4 glycoprotein superfamily, interacts with integrins in the presence of ameloblastin. Ameloblastin bound to CD63 and promoted CD63 binding to integrin β1. The interaction between CD63 and integrin β1 induced Src kinase inactivation via the binding of CD63 to Src. The reduction of Src activity and osteogenic differentiation mediated by ameloblastin were abrogated by treatment with anti-CD63 antibody and overexpression of constitutively active Src, respectively. Therefore, our results suggest that ameloblastin is expressed in osteoblasts and functions as a promoting factor for osteogenic differentiation via a novel pathway through the interaction between CD63 and integrin β1.
|
| 巻・号 |
31(4)
|
| ページ |
783-92
|
| 公開日 |
2011-2-1
|
| DOI |
10.1128/MCB.00912-10
|
| PII |
MCB.00912-10
|
| PMID |
21149578
|
| PMC |
PMC3028634
|
| MeSH |
Animals
Antigens, CD / genetics
Antigens, CD / metabolism*
Base Sequence
Cell Line
Cell Line, Tumor
Cells, Cultured
DNA Primers / genetics
Dental Enamel Proteins / genetics
Dental Enamel Proteins / metabolism*
Humans
Integrin beta1 / metabolism*
Minerals / metabolism
Models, Biological
Osteoblasts / cytology
Osteoblasts / metabolism
Osteogenesis / physiology*
Osteosarcoma / genetics
Osteosarcoma / metabolism
Osteosarcoma / pathology
Platelet Membrane Glycoproteins / antagonists & inhibitors
Platelet Membrane Glycoproteins / genetics
Platelet Membrane Glycoproteins / metabolism*
RNA, Small Interfering / genetics
Rats
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Signal Transduction
Tetraspanin 30
src-Family Kinases / antagonists & inhibitors*
src-Family Kinases / metabolism
|
| IF |
3.611
|
| 引用数 |
37
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
NOS-1(RCB1032)
Saos-2(RCB0428)
MG-63(RCB1890)
HOS(RCB0992) |
