| RRC ID |
18244
|
| 著者 |
Yang CL, Chen HW, Wang TC, Wang YJ.
|
| タイトル |
A novel fibrin gel derived from hyaluronic acid-grafted fibrinogen.
|
| ジャーナル |
Biomed Mater
|
| Abstract |
Fibrinogen is a major plasma protein that forms a three-dimensional fibrin gel upon being activated by thrombin. In this study, we report the synthesis and potential applications of hybrid molecules composed of fibrinogen coupled to the reducing ends of short-chain hyaluronic acids (sHAs) by reductive amination. The grafting of sHAs to fibrinogen was verified by analyzing particle size, zeta potential and gel-electrophoretic mobility of the hybrid molecules. The sHA-fibrinogen hybrid molecules with graft ratios (sHA/fibrinogen) of up to 6.5 retained the ability to form gels in response to thrombin activation. The sHA-fibrin gels were transparent in appearance and exhibited high water content, which were characteristics distinct from those of gels formed by mixtures of sHAs and fibrinogen. The potential applications of the sHA-fibrin gels were evaluated. The sHA-fibrinogen gel with a graft ratio of 3.6 (S3.6F) was examined for its ability to encapsulate and support the differentiation of ATDC5 chondrocyte-like cells. Compared with the fibrinogen-formed gel, cells cultured in the S3.6F gel exhibited increased lacunae formation; moreover, the abundance of cartilaginous extracellular matrix molecules and the expression of chondrocyte marker genes, such as aggrecan, collagen II and Sox9, were also significantly increased. Our data suggest that the three-dimensional gel formed by the sHA-fibrinogen hybrid is a better support than the fibrin gel for chondrogenesis induction.
|
| 巻・号 |
6(2)
|
| ページ |
025009
|
| 公開日 |
2011-4-1
|
| DOI |
10.1088/1748-6041/6/2/025009
|
| PII |
S1748-6041(11)74359-5
|
| PMID |
21393811
|
| MeSH |
Animals
Biocompatible Materials / chemistry
Blood Proteins / chemistry
Cartilage / metabolism
Cell Differentiation
Cell Line
Chondrocytes / cytology
Extracellular Matrix / metabolism
Fibrin / chemistry*
Fibrinogen / chemistry*
Gels / chemistry
Hyaluronic Acid / chemistry*
Mice
Microscopy, Electron, Scanning
Models, Chemical
Particle Size
Thrombin / chemistry
Water / chemistry
|
| IF |
3.174
|
| 引用数 |
10
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
ATDC5(RCB0565) |
