| RRC ID |
18621
|
| 著者 |
Haniu H, Saito N, Matsuda Y, Kim YA, Park KC, Tsukahara T, Usui Y, Aoki K, Shimizu M, Ogihara N, Hara K, Takanashi S, Okamoto M, Ishigaki N, Nakamura K, Kato H.
|
| タイトル |
Effect of dispersants of multi-walled carbon nanotubes on cellular uptake and biological responses.
|
| ジャーナル |
Int J Nanomedicine
|
| Abstract |
Although there have been many reports about the cytotoxicity of multi-walled carbon nanotubes (MWCNTs), the results are still controversial. To investigate one possible reason, the authors investigated the influence of MWCNT dispersants on cellular uptake and cytotoxicity. Cytotoxicity was examined (measured by alamarBlue(®) assay), as well as intracellular MWCNT concentration and cytokine secretion (measured by flow cytometry) in human bronchial epithelial cells (BEAS-2B) exposed to a type of highly purified MWCNT vapor grown carbon fiber (VGCF(®), Shōwa Denkō Kabushiki-gaisha, Tokyo, Japan) in three different dispersants (gelatin, carboxylmethyl cellulose, and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine). The authors also researched the relationship between the intracellular concentration of MWCNTs and cytotoxicity by using two cell lines, BEAS-2B and MESO-1 human malignant pleural mesothelioma cells. The intracellular concentration of VGCF was different for each of the three dispersants, and the levels of cytotoxicity and inflammatory response were correlated with the intracellular concentration of VGCF. A relationship between the intracellular concentration of VGCF and cytotoxic effects was observed in both cell lines. The results indicate that dispersants affect VGCF uptake into cells and that cytotoxicity depends on the intracellular concentration of VGCF, not on the exposed dosage. Thus, toxicity appears to depend on exposure time, even at low VGCF concentrations, because VGCF is biopersistent.
|
| 巻・号 |
6
|
| ページ |
3295-307
|
| 公開日 |
2011-1-1
|
| DOI |
10.2147/IJN.S26573
|
| PII |
ijn-6-3295
|
| PMID |
22228997
|
| PMC |
PMC3252677
|
| MeSH |
Apoptosis / drug effects
Cell Line
Cell Survival / drug effects
Diffusion
Epithelial Cells / chemistry*
Epithelial Cells / cytology
Epithelial Cells / drug effects*
Humans
Materials Testing
Nanotubes, Carbon / chemistry*
Nanotubes, Carbon / toxicity*
Nanotubes, Carbon / ultrastructure
Particle Size
|
| IF |
5.115
|
| 引用数 |
41
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
NANOSCIENCE & NANOTECHNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
ACC-MESO-1(RCB2292) |
