論文 - 詳細
| RRC ID | 18726 |
|---|---|
| 著者 | Maeda H, Nakano T, Tomokiyo A, Fujii S, Wada N, Monnouchi S, Hori K, Akamine A. |
| タイトル | Mineral trioxide aggregate induces bone morphogenetic protein-2 expression and calcification in human periodontal ligament cells. |
| ジャーナル | J Endod |
| Abstract |
INTRODUCTION:Mineral trioxide aggregate (MTA) is a therapeutic, endodontic repair material that is reported to exhibit calcified tissue-conductive activity although the mechanisms remain unclear. We hypothesize that the dissolution of calcium from MTA into the surrounding environment may play an important role in the osteoblastic/cementoblastic differentiation of human periodontal ligament cells (HPLCs). METHODS:Two populations of HPLCs were obtained from two patients, respectively, and were cultured in the presence or absence of MTA discs and/or CaCl(2) in order to investigate calcium release, calcification activity, calcium-sensing receptor (CaSR) gene expression and bone morphogenetic protein-2 (BMP-2), and BMP-2 receptor protein and gene expression. RESULTS:MTA released a substantial accumulation of calcium (4 mmol/L) within 14 days into culture media. After 4 weeks, the two populations of HPLCs independently exhibited calcification as well as BMP-2 distribution in the vicinity of MTA. HPLCs inherently expressed genes encoding for the CaSR and BMP-2 receptors. Exogenous CaCl(2) media supplementation induced CaSR gene expression in HPLCs and calcification and BMP-2 synthesis throughout the entire HPLC cultures, whereas MgCl(2) had no effect. Both MTA and CaCl(2) stimulated BMP-2 gene expression above that of baseline levels. CONCLUSION:Here we show the first report showing that HPLCs cocultured directly with MTA up-regulated BMP2 expression and calcification. These results may be through CaSR interactions that were potentially activated by the release of calcium from MTA into the culture environment. |
| 巻・号 | 36(4) |
| ページ | 647-52 |
| 公開日 | 2010-4-1 |
| DOI | 10.1016/j.joen.2009.12.024 |
| PII | S0099-2399(09)01101-7 |
| PMID | 20307738 |
| MeSH | Adolescent Aluminum Compounds / metabolism Aluminum Compounds / pharmacology* Bone Morphogenetic Protein 2 / biosynthesis* Calcium / metabolism Calcium Chloride / pharmacology Calcium Compounds / metabolism Calcium Compounds / pharmacology* Cell Differentiation / drug effects Cells, Cultured Cementogenesis / drug effects Coculture Techniques Dental Cementum / cytology Drug Combinations Female Gene Expression / drug effects Humans Male Osteoblasts / cytology Osteogenesis / drug effects Oxides / metabolism Oxides / pharmacology* Periodontal Ligament / cytology Periodontal Ligament / drug effects* Periodontal Ligament / metabolism* Receptors, Calcium-Sensing / biosynthesis Reverse Transcriptase Polymerase Chain Reaction Root Canal Filling Materials / metabolism Root Canal Filling Materials / pharmacology* Silicates / metabolism Silicates / pharmacology* Tooth Calcification / drug effects* Young Adult |
| IF | 3.118 |
| 引用数 | 60 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| リソース情報 | |
| ヒト・動物細胞 | NHSF46(RCB0162) |