RRC ID |
1898
|
著者 |
Cao F, Hata R, Zhu P, Ma YJ, Tanaka J, Hanakawa Y, Hashimoto K, Niinobe M, Yoshikawa K, Sakanaka M.
|
タイトル |
Overexpression of SOCS3 inhibits astrogliogenesis and promotes maintenance of neural stem cells.
|
ジャーナル |
J Neurochem
|
Abstract |
To investigate the effects of suppressors of cytokine signaling 3 (SOCS3) on neural stem cell fate, stem cells were infected with an adenoviral vector expressing SOCS3. Three days later, western blot analysis and immunocytochemical analysis revealed that the protein level of MAP2 and the number of MAP2-positive cells were significantly increased in SOCS3-transfected cells, whereas the protein level of GFAP and the number of GFAP-positive cells were significantly decreased. Furthermore, promoter assay revealed a significant reduction in the transcriptional level of signal transducer and activator of transcription 3 (Stat3) in the transfected cells. In addition, the mRNA levels of Notch family member (notch1) and inhibitory basic helix-loop-helix (bHLH) factors (hes5 and id3) were significantly up-regulated 1 day after overexpression of SOCS3. Three days after transfection, the mRNA level of hes5 was significantly decreased, whereas that of notch1 was still up-regulated. Moreover, all of SOCS3-positive cells expressed Nestin protein but did not express MAP2 or GFAP proteins. These data indicate that overexpression of SOCS3 induced neurogenesis and inhibited astrogliogenesis in neural stem cells. Our data also show that SOCS3 promoted maintenance of neural stem cells.
|
巻・号 |
98(2)
|
ページ |
459-70
|
公開日 |
2006-7-1
|
DOI |
10.1111/j.1471-4159.2006.03890.x
|
PII |
JNC3890
|
PMID |
16805839
|
MeSH |
Adenoviridae / metabolism
Astrocytes / drug effects*
Cell Differentiation / drug effects
Genetic Vectors
Glial Fibrillary Acidic Protein / biosynthesis
Helix-Loop-Helix Motifs / genetics
Humans
Immunoblotting
Immunohistochemistry
Leukocyte Migration-Inhibitory Factors / pharmacology
Luciferases / metabolism
Neurons / drug effects*
Promoter Regions, Genetic / genetics
Receptor, Notch1 / biosynthesis
Receptor, Notch1 / genetics
Reverse Transcriptase Polymerase Chain Reaction
STAT3 Transcription Factor / physiology
Signal Transduction / physiology
Stem Cells / drug effects*
Suppressor of Cytokine Signaling 3 Protein
Suppressor of Cytokine Signaling Proteins / biosynthesis*
Suppressor of Cytokine Signaling Proteins / genetics*
Up-Regulation
|
IF |
4.066
|
引用数 |
29
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
NEUROSCIENCES
|
リソース情報 |
遺伝子材料 |
pAxCAwt (RDB1678) |