| RRC ID |
19156
|
| 著者 |
Sato N, Morimoto H, Baba R, Nakamata J, Doi Y, Yamaguchi K.
|
| タイトル |
Functional expression of double-stranded RNA-dependent protein kinase in rat intestinal epithelial cells.
|
| ジャーナル |
J Cell Biochem
|
| Abstract |
Intestinal epithelial cells (IECs) are exposed to external environment, microbial and viral products, and serve as essential barriers to antigens. Recent studies have shown that IECs express Toll-like receptors (TLRs) and respond to microbial components. The antimicrobial and antiviral barriers consist of many molecules including TLRs. To investigate the further component of this barrier in intestine, we examined the expression of double-stranded RNA-dependent protein kinase (PKR). PKR is a player in the cellular antiviral response and phosphorylates alpha-subunit of the eukaryotic translation initiation factor 2 (eIF-2alpha) to block protein synthesis and induces apoptosis. In this study, we showed that the expression of PKR was restricted to the cytoplasm of absorptive epithelial cells in the intestine of adult rat. We also demonstrated that PKR was expressed in the cultured rat intestinal epithelial cells (IEC-6). The level of PKR protein expression and the activity of alkaline phosphatase (ALP) increased in the cultured IEC-6 cells in a time-dependent manner. Inhibition of PKR by the 2-aminopurine treatment decreased ALP activity in the IEC-6 cells. Treatment of IEC-6 cells with synthetic double-stranded RNA (dsRNA) induced cell death in a dose-dependent manner. The addition of hydrocortisone also provoked suppression of PKR expression and ALP activity. This modulation might be mediated by signal transducers and activators of transcription-1 (STAT-1) protein. We concluded that PKR is expressed in IECs as potent barriers to antigens and is a possible modulator of the differentiation of rat IECs.
|
| 巻・号 |
110(1)
|
| ページ |
104-11
|
| 公開日 |
2010-5-1
|
| DOI |
10.1002/jcb.22513
|
| PMID |
20213745
|
| MeSH |
Alkaline Phosphatase / metabolism
Animals
Cells, Cultured
Enterocytes / cytology
Enterocytes / drug effects
Enterocytes / enzymology*
Gene Expression Regulation, Enzymologic / drug effects
Hydrocortisone / pharmacology
Intestine, Small / cytology
Intestine, Small / drug effects
Intestine, Small / enzymology
Poly I-C / pharmacology
Protein Kinase Inhibitors / pharmacology
Protein Transport / drug effects
RNA, Double-Stranded / pharmacology
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rats
Rats, Inbred BN
STAT1 Transcription Factor / metabolism
Subcellular Fractions / drug effects
Subcellular Fractions / enzymology
eIF-2 Kinase / genetics*
eIF-2 Kinase / metabolism*
|
| IF |
4.237
|
| 引用数 |
4
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
IEC 6(RCB0993) |
