| RRC ID |
19230
|
| 著者 |
Kawai H, Yamashita T, Ohta Y, Deguchi K, Nagotani S, Zhang X, Ikeda Y, Matsuura T, Abe K.
|
| タイトル |
Tridermal tumorigenesis of induced pluripotent stem cells transplanted in ischemic brain.
|
| ジャーナル |
J Cereb Blood Flow Metab
|
| Abstract |
Stroke is a major neurologic disorder. Induced pluripotent stem (iPS) cells can be produced from basically any part of patients, with high reproduction ability and pluripotency to differentiate into various types of cells, suggesting that iPS cells can provide a hopeful therapy for cell transplantation. However, transplantation of iPS cells into ischemic brain has not been reported. In this study, we showed that the iPS cells fate in a mouse model of transient middle cerebral artery occlusion (MCAO). Undifferentiated iPS cells (5 x 10(5)) were transplanted into ipsilateral striatum and cortex at 24 h after 30 mins of transient MCAO. Behavioral and histologic analyses were performed at 28 day after the cell transplantation. To our surprise, the transplanted iPS cells expanded and formed much larger tumors in mice postischemic brain than in sham-operated brain. The clinical recovery of the MCAO+iPS group was delayed as compared with the MCAO+PBS (phosphate-buffered saline) group. iPS cells formed tridermal teratoma, but could supply a great number of Dcx-positive neuroblasts and a few mature neurons in the ischemic lesion. iPS cells have a promising potential to provide neural cells after ischemic brain injury, if tumorigenesis is properly controlled.
|
| 巻・号 |
30(8)
|
| ページ |
1487-93
|
| 公開日 |
2010-8-1
|
| DOI |
10.1038/jcbfm.2010.32
|
| PII |
jcbfm201032
|
| PMID |
20216552
|
| PMC |
PMC2949240
|
| MeSH |
Animals
Brain / cytology
Brain / pathology*
Brain / surgery
Brain Ischemia / pathology
Brain Ischemia / surgery
Brain Ischemia / therapy
Cell Survival
Doublecortin Protein
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / pathology
Induced Pluripotent Stem Cells / transplantation*
Infarction, Middle Cerebral Artery / pathology
Infarction, Middle Cerebral Artery / surgery*
Infarction, Middle Cerebral Artery / therapy
Male
Mice
Mice, Inbred C57BL
Motor Activity
Neoplastic Processes
Neurogenesis
|
| IF |
5.681
|
| 引用数 |
132
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
NEUROSCIENCES
HEMATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
iPS-MEF-Ng-20D-17(APS0001) |
