| RRC ID |
19672
|
| 著者 |
Kubo A, Suzuki N, Yuan X, Nakai K, Satoh N, Imai KS, Satou Y.
|
| タイトル |
Genomic cis-regulatory networks in the early Ciona intestinalis embryo.
|
| ジャーナル |
Development
|
| Abstract |
Precise spatiotemporal gene expression during animal development is achieved through gene regulatory networks, in which sequence-specific transcription factors (TFs) bind to cis-regulatory elements of target genes. Although numerous cis-regulatory elements have been identified in a variety of systems, their global architecture in the gene networks that regulate animal development is not well understood. Here, we determined the structure of the core networks at the cis-regulatory level in early embryos of the chordate Ciona intestinalis by chromatin immunoprecipitation (ChIP) of 11 TFs. The regulatory systems of the 11 TF genes examined were tightly interconnected with one another. By combining analysis of the ChIP data with the results of previous comprehensive analyses of expression profiles and knockdown of regulatory genes, we found that most of the previously determined interactions are direct. We focused on cis-regulatory networks responsible for the Ciona mesodermal tissues by examining how the networks specify these tissues at the level of their cis-regulatory architecture. We also found many interactions that had not been predicted by simple gene knockdown experiments, and we showed that a significant fraction of TF-DNA interactions make major contributions to the regulatory control of target gene expression.
|
| 巻・号 |
137(10)
|
| ページ |
1613-23
|
| 公開日 |
2010-5-1
|
| DOI |
10.1242/dev.046789
|
| PII |
dev.046789
|
| PMID |
20392745
|
| MeSH |
Animals
Animals, Genetically Modified
Body Patterning / genetics
Chromatin Immunoprecipitation
Chromosome Mapping
Ciona intestinalis / embryology*
Ciona intestinalis / genetics*
Embryo, Nonmammalian
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Regulatory Networks / physiology*
Models, Biological
Oligonucleotide Array Sequence Analysis
Protein Binding / physiology
Regulatory Sequences, Nucleic Acid / genetics
Regulatory Sequences, Nucleic Acid / physiology*
Time Factors
Transcription Factors / metabolism
|
| IF |
5.611
|
| 引用数 |
48
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| カタユウレイボヤ・(ニッポンウミシダ) |
Wild C. int |
