| RRC ID |
19703
|
| 著者 |
Danilova TV, Friebe B, Gill BS.
|
| タイトル |
Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat.
|
| ジャーナル |
Chromosoma
|
| Abstract |
Fluorescence in situ hybridization (FISH) is a useful tool for physical mapping of chromosomes and studying evolutionary chromosome rearrangements. Here we report a robust method for single-copy gene FISH for wheat. FISH probes were developed from cDNA of cytosolic acetyl-CoA carboxylase (ACCase) gene (Acc-2) and mapped on chromosomes of bread wheat, Triticum aestivum L. (2n = 6x = 42, AABBDD), and related diploid and tetraploid species. Another nine full-length (FL) cDNA FISH probes were mapped and used to identify chromosomes of wheat species. The Acc-2 probe was detected on the long arms of each of the homoeologous group 3 chromosomes (3A, 3B, and 3D), on 5DL and 4AL of bread wheat, and on homoeologous and nonhomoeologous chromosomes of other species. In the species tested, FISH detected more Acc-2 gene or pseudogene sites than previously found by PCR and Southern hybridization analyses and showed presence/absence polymorphism of Acc-2 sequences. FISH with the Acc-2 probe revealed the 4A-5A translocation, shared by several related diploid and polyploid species and inherited from an ancestral A-genome species, and the T. timopheevii-specific 4A(t)-3A(t) translocation.
|
| 巻・号 |
121(6)
|
| ページ |
597-611
|
| 公開日 |
2012-12-1
|
| DOI |
10.1007/s00412-012-0384-7
|
| PMID |
23052335
|
| MeSH |
Acetyl-CoA Carboxylase / genetics*
Chromosome Mapping*
Chromosomes, Plant
DNA, Complementary
Diploidy
Genome, Plant*
In Situ Hybridization, Fluorescence / methods*
Plant Proteins / genetics
Tetraploidy
Triticum / genetics*
|
| IF |
3.442
|
| 引用数 |
53
|
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| コムギ |
tplb0013a02
tplb0029f23
tplb0048d21
tplb0011e24
tplb0004j16
tplb0017g02
tplb0033b21
tplb0043p15
tplb0027f03 |
