| RRC ID |
20081
|
| 著者 |
Robertson BR, Tezuka N, Watanabe MM.
|
| タイトル |
Phylogenetic analyses of Synechococcus strains (cyanobacteria) using sequences of 16S rDNA and part of the phycocyanin operon reveal multiple evolutionary lines and reflect phycobilin content.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
The genus Synechococcus (cyanobacteria), while containing morphologically similar isolates, is polyphyletic and organisms presently classified as such require reclassification into several independent genera. Studies based on analysis of 16S rRNA gene sequences have shown that members of the genus Synechococcus are affiliated to three of seven deeply branching cyanobacterial lineages. In addition, some strains do not appear to be associated with any of these lineages and may represent novel clades. In this report, a cyanobacterial phylogeny based on 16S rDNA sequences, including 14 newly sequenced Synechococcus isolates, is presented. One newly sequenced Synechococcus strain (PCC 7902) did not have any close relatives amongst cyanobacterial isolates currently contained in 16S rDNA sequence databases and was only loosely affiliated to a cyanobacterial lineage in which no other Synechococcus strains were found. Three hot-spring Synechococcus isolates, including two that were newly sequenced in this study (PCC 6716 and PCC 6717), formed an additional cyanobacterial lineage. These results indicated that Synechococcus species are affiliated to five of eight deeply branching cyanobacterial lineages. Part of the phycocyanin (PC) gene sequence (cpc), including the intergenic spacer (IGS) between cpcB and cpcA and the corresponding flanking regions (cpcBA-IGS), was used to investigate relationships between closely related Synechococcus isolates. Previously described PCR primers did not amplify this region from the majority of strains under investigation, so a new set of primers was designed that allowed amplification and sequencing of the cpcBA-IGS and flanking regions from 38 Synechococcus species. Phylogenetic analysis of this region was largely consistent with that obtained from 16S rDNA sequence analysis and revealed a relationship between the primary PC DNA sequence and the phycobilin content of cells.
|
| 巻・号 |
51(Pt 3)
|
| ページ |
861-871
|
| 公開日 |
2001-5-1
|
| DOI |
10.1099/00207713-51-3-861
|
| PMID |
11411708
|
| MeSH |
Base Sequence
Cyanobacteria / classification*
Cyanobacteria / genetics*
DNA Primers
DNA, Ribosomal / genetics*
Evolution, Molecular*
Molecular Sequence Data
Operon*
Phycobilins
Phycocyanin / genetics*
Phycocyanin / metabolism
Phylogeny*
Polymerase Chain Reaction
Pyrroles / metabolism
RNA, Ribosomal, 16S / genetics*
Tetrapyrroles
|
| IF |
2.415
|
| 引用数 |
136
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 藻類 |
NIES-937
NIES-938
NIES-939
NIES-940
NIES-941
NIES-942
NIES-943
NIES-944
NIES-946
NIES-947
NIES-948
NIES-949
NIES-950
NIES-952
NIES-954
NIES-955
NIES-956
NIES-958
NIES-960
NIES-961
NIES-962
NIES-963
NIES-964
NIES-965
NIES-951
NIES-945
NIES-953
NIES-957
NIES-959 |
