論文 - 詳細
| RRC ID | 20978 |
|---|---|
| 著者 | Kovacikova I, Polakova S, Benko Z, Cipak L, Zhang L, Rumpf C, Miadokova E, Gregan J. |
| タイトル | A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe. |
| ジャーナル | Cell Cycle |
| Abstract |
The reduction of chromosome number during meiosis is achieved by two successive rounds of chromosome segregation after just single round of DNA replication. To identify novel proteins required for the proper segregation of chromosomes during meiosis, we analyzed the consequences of deleting Schizosaccharomyces pombe genes predicted to encode protein kinases that are not essential for cell viability. We show that Mph1, a member of the Mps1 family of spindle assembly checkpoint kinases, is required to prevent meiosis I homolog non-disjunction. We also provide evidence for a novel function of Spo4, the fission yeast ortholog of Dbf4-dependent Cdc7 kinase, in regulating the length of anaphase II spindles. In the absence of Spo4, abnormally elongated anaphase II spindles frequently overlap and thus destroy the linear order of nuclei in the ascus. Our observation that the spo4Δ mutant phenotype can be partially suppressed by inhibiting Cdc2-as suggests that dysregulation of the activity of this cyclin-dependent kinase may cause abnormal elongation of anaphase II spindles in spo4Δ mutant cells. |
| 巻・号 | 12(4) |
| ページ | 618-24 |
| 公開日 | 2013-2-15 |
| DOI | 10.4161/cc.23513 |
| PII | 23513 |
| PMID | 23370392 |
| PMC | PMC3594262 |
| MeSH | CDC2 Protein Kinase / antagonists & inhibitors CDC2 Protein Kinase / genetics CDC2 Protein Kinase / metabolism Cell Cycle Proteins / deficiency Cell Cycle Proteins / genetics Cell Nucleus / genetics Cell Nucleus / metabolism Chromosomes, Fungal / genetics* Chromosomes, Fungal / ultrastructure DNA Replication Gene Expression Regulation, Fungal* Gene Knockout Techniques Genotype Meiosis / genetics* Nondisjunction, Genetic* Phenotype Plasmids / genetics Protein Kinases / genetics Protein Kinases / metabolism Protein Serine-Threonine Kinases / deficiency Protein Serine-Threonine Kinases / genetics Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / antagonists & inhibitors Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism |
| IF | 3.699 |
| 引用数 | 11 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 酵母 | |