RRC ID |
21397
|
著者 |
Tsuda T, Suzuki T, Kojima S.
|
タイトル |
Crystallization and preliminary X-ray diffraction analysis of Bacillus subtilis YwfE, an L-amino-acid ligase.
|
ジャーナル |
Acta Crystallogr Sect F Struct Biol Cryst Commun
|
Abstract |
Bacillus subtilis YwfE, an L-amino-acid ligase, catalyzes the formation of an α-dipeptide from L-amino acids in an ATP-dependent manner. In order to elucidate the substrate-recognition mode and the reaction mechanism of this ligase, native and selenomethionine-derivatized (SeMet) crystals of YwfE in the presence of ADP, MgCl(2) and the dipeptide L-Ala-L-Gln were obtained using the hanging-drop vapour-diffusion method. These crystals diffracted to 1.9 and 2.8 Å resolution, respectively. Preliminary SAD phase calculations using the data set from the SeMet crystal suggested that the crystal belonged to the hexagonal space group P6(5)22, with unit-cell parameters a = b = 90.85, c = 250.31 Å, and contained one molecule in the asymmetric unit with a solvent content of 57.3%.
|
巻・号 |
68(Pt 2)
|
ページ |
203-6
|
公開日 |
2012-2-1
|
DOI |
10.1107/S174430911105425X
|
PII |
S174430911105425X
|
PMID |
22298000
|
PMC |
PMC3274404
|
MeSH |
Bacillus subtilis / enzymology*
Crystallization
Crystallography, X-Ray
Peptide Synthases / chemistry*
|
IF |
0.968
|
引用数 |
3
|
WOS 分野
|
CRYSTALLOGRAPHY
BIOPHYSICS
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
一般微生物 |
JCM 1465 |