論文 - 詳細
| RRC ID | 21453 |
|---|---|
| 著者 | Xu FX, Xu YP, Jin LJ, Liu H, Wang LH, You JS, Li SY, Li XY. |
| タイトル | Effectiveness of egg yolk immunoglobulin (IgY) against periodontal disease-causing Fusobacterium nucleatum. |
| ジャーナル | J Appl Microbiol |
| Abstract |
AIMS:To evaluate the in vitro and in vivo effectiveness of egg yolk immunoglobulin (IgY) against periodontal disease-causing Fusobacterium nucleatum. METHODS AND RESULTS:Four White Leghorn hens (120 days old) were immunized with whole Fus. nucleatum cells killed with 1% formaldehyde using three injections provided at 2-week intervals. IgY was produced from egg yolks obtained from these immunized hens using water dilution, two-step salt precipitation and ultrafiltration. This IgY was shown to have a purity of 86·8% based on its optical intensity in the stained SDS-PAGE bands. An enzyme-linked immunosorbent assay indicated a high specificity for the IgY against Fus. nucleatum with a maximum antibody titre of 80 000. The IgY had only weak cross-reactivity with Porphyromonas gingivalis, Prevotella intermedia and Solobacterium moorei. Growth and biofilm formation by Fus. nucleatum were inhibited by IgY at concentrations of 10 and 20 mg ml(-1) . Immunofluorescence and immunoelectron microscope assays revealed a high binding ability of specific IgY, which may explain the in vitro effectiveness of IgY. In an in vivo study, IgY treatment resulted in a marked decrease in alveolar bone loss after Fus. nucleatum infection in a mouse model confirming the effectiveness of IgY against periodontal disease-causing Fus. nucleatum. CONCLUSIONS:IgY effectively inhibited growth and biofilm formation by Fus. nucleatum and prevented the progression of periodontal disease by decreasing alveolar bone loss. SIGNIFICANCE AND IMPACT OF THE STUDY:Specific IgY may have potential for the treatment of periodontal disease. |
| 巻・号 | 113(4) |
| ページ | 983-91 |
| 公開日 | 2012-10-1 |
| DOI | 10.1111/j.1365-2672.2012.05396.x |
| PMID | 22789022 |
| MeSH | Alveolar Bone Loss / prevention & control* Animals Antibodies, Bacterial / isolation & purification Antibodies, Bacterial / pharmacology Antibody Specificity Biofilms / drug effects* Biofilms / growth & development Chickens Cross Reactions Egg Yolk / immunology Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Female Fusobacterium nucleatum / drug effects* Fusobacterium nucleatum / growth & development Immunoglobulins / isolation & purification Immunoglobulins / pharmacology* Male Mice |
| IF | 3.066 |
| 引用数 | 8 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 10645 JCM 11024 JCM 12248 JCM 12257 |