RRC ID |
21784
|
著者 |
Ohtsuka H, Ogawa S, Kawamura H, Sakai E, Ichinose K, Murakami H, Aiba H.
|
タイトル |
Screening for long-lived genes identifies Oga1, a guanine-quadruplex associated protein that affects the chronological lifespan of the fission yeast Schizosaccharomyces pombe.
|
ジャーナル |
Mol Genet Genomics
|
Abstract |
Schizosaccharomyces pombe and Saccharomyces cerevisiae are excellent model organisms to study lifespan. We conducted screening to identify novel genes that, when overexpressed, extended the chronological lifespan of fission yeast. We identified seven genes, among which we focused on SPBC16A3.08c. The gene product showed similarity to Ylr150w of S. cerevisiae, which has affinity for guanine-quadruplex nucleic acids (G4). The SPBC16A3.08c product associated with G4 in vitro and complemented the phenotype of an S. cerevisiae Ylr150w deletion mutant. From these results, we proposed that SPBC16A3.08c encoded for a functional homolog of Ylr150w, which we designated ortholog of G4-associated protein (oga1 (+)). oga1 (+) overexpression extended the chronological lifespan and also decreased mating efficiency and caused both high and low temperature-sensitive growth. Deleting oga1 (+) resulted in caffeine-sensitive and canavanine-resistant phenotypes. Based on these results, we discuss the function of Oga1 on the chronological lifespan of fission yeast.
|
巻・号 |
288(5-6)
|
ページ |
285-95
|
公開日 |
2013-6-1
|
DOI |
10.1007/s00438-013-0748-6
|
PMID |
23640107
|
MeSH |
Caffeine / pharmacology
Canavanine / pharmacology
Cloning, Molecular
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism
Drug Resistance, Fungal / genetics
G-Quadruplexes*
Gene Deletion
Gene Expression Regulation, Fungal
Genetic Complementation Test
Protein Kinases / genetics
Saccharomyces cerevisiae / drug effects
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae Proteins / genetics
Schizosaccharomyces / drug effects
Schizosaccharomyces / physiology*
Schizosaccharomyces pombe Proteins / genetics*
Schizosaccharomyces pombe Proteins / metabolism
|
IF |
2.797
|
引用数 |
12
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
酵母 |
BY23849(BY4741) |