RRC ID 21807
Author Yamaoka T, Imada K, Fukunishi K, Yamasaki Y, Shimoda C, Nakamura T.
Title The fission yeast synaptobrevin ortholog Syb1 plays an important role in forespore membrane formation and spore maturation.
Journal Eukaryot Cell
Abstract Synaptobrevin, also called vesicle-associated membrane protein (VAMP), is a component of the plasma membrane N-methylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, which plays a key role in intracellular membrane fusion. Previous studies have revealed that, similar to synaptobrevin in other organisms, the fission yeast synaptobrevin ortholog Syb1 associates with post-Golgi secretory vesicles and is essential for cytokinesis and cell elongation. Here, we report that Syb1 has a role in sporulation. After nitrogen starvation, green fluorescent protein (GFP)-Syb1 is found in intracellular dots. As meiosis proceeds, GFP-Syb1 accumulates around the nucleus and then localizes at the forespore membrane (FSM). We isolated a syb-S1 mutant, which exhibits a defect in sporulation. In syb1-S1 mutants, the FSM begins to form but fails to develop a normal morphology. Electron microscopy shows that an abnormal spore wall is often formed in syb1-S1 mutant spores. Although most syb1-S1 mutant spores are germinated, they are less tolerant to ethanol than wild-type spores. The syb1-S1 allele carries a missense mutation, resulting in replacement of a conserved cysteine residue adjacent to the transmembrane domain, which reduces the stability and abundance of the Syb1 protein. Taken together, these results indicate that Syb1 plays an important role in both FSM assembly and spore wall formation.
Volume 12(9)
Pages 1162-70
Published 2013-9-1
DOI 10.1128/EC.00061-13
PII EC.00061-13
PMID 23709180
PMC PMC3811565
MeSH Cell Membrane / metabolism* Cell Membrane / ultrastructure Cell Nucleus / metabolism Cell Wall / metabolism Cell Wall / ultrastructure Ethanol / pharmacology Meiosis Mutation, Missense Protein Structure, Tertiary R-SNARE Proteins / chemistry R-SNARE Proteins / genetics R-SNARE Proteins / metabolism* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces / physiology Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spores, Fungal / drug effects Spores, Fungal / metabolism* Spores, Fungal / physiology Spores, Fungal / ultrastructure
IF 2.992
Times Cited 3
WOS Category MYCOLOGY MICROBIOLOGY
Resource
Yeast FY17356(TY1) FY17360(TY5) FY17418(TY58) FY17488(TY128) FY17513(TY149) FY17525(TY161) FY17571(TY208) FY17572(TY209) FY17585(TY222) FY17590(TY227) FY17592(TY229) FY17601(TY234) FY17602(TY235) FY17612(TY245) FY17613(TY246) FY7456(MKW5)