RRC ID |
21864
|
著者 |
Baek SH, Cho HW, Kwon YC, Lee JH, Kim MJ, Lee H, Choe KM.
|
タイトル |
Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing.
|
ジャーナル |
FEBS Lett
|
Abstract |
Rho-family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in wound closure of the Drosophila larval epidermis. Pak3-deficient larvae failed to close a wound hole and this defect was not rescued by Pak1 expression, indicating differential functions of the two proteins. Pak3 localized to the wound margin, which selectively required Rac1. Pak3-deficient larvae showed severe defects in actin-myosin organization at the wound margin and in submarginal cells, which was reminiscent of the phenotypes of Rac1-deficient larvae. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.
|
巻・号 |
586(6)
|
ページ |
772-7
|
公開日 |
2012-3-23
|
DOI |
10.1016/j.febslet.2012.01.061
|
PII |
S0014-5793(12)00110-X
|
PMID |
22449966
|
MeSH |
Actins / metabolism*
Animals
Drosophila Proteins / metabolism*
Drosophila melanogaster / physiology*
Epidermis / pathology
Epidermis / physiology
Larva / anatomy & histology
Larva / physiology*
Myosins / metabolism*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Wound Healing / physiology
p21-Activated Kinases / genetics
p21-Activated Kinases / metabolism*
rac1 GTP-Binding Protein / genetics
rac1 GTP-Binding Protein / metabolism*
|
IF |
3.057
|
引用数 |
14
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
ショウジョウバエ |
14895R-1
2248R-2
9774R-2
18255R-3 |