| RRC ID |
21865
|
| 著者 |
Sakaidani Y, Ichiyanagi N, Saito C, Nomura T, Ito M, Nishio Y, Nadano D, Matsuda T, Furukawa K, Okajima T.
|
| タイトル |
O-linked-N-acetylglucosamine modification of mammalian Notch receptors by an atypical O-GlcNAc transferase Eogt1.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
O-linked-β-N-acetylglucosamine (O-GlcNAc) modification is a unique cytoplasmic and nuclear protein modification that is common in nearly all eukaryotes, including filamentous fungi, plants, and animals. We had recently reported that epidermal growth factor (EGF) repeats of Notch and Dumpy are O-GlcNAcylated by an atypical O-GlcNAc transferase, EOGT, in Drosophila. However, no study has yet shown whether O-GlcNAcylation of extracellular proteins is limited to insects such as Drosophila or whether it occurs in other organisms, including mammals. Here, we report the characterization of A130022J15Rik, a mouse gene homolog of Drosophila Eogt (Eogt 1). Enzymatic analysis revealed that Eogt1 has a substrate specificity similar to that of Drosophila EOGT, wherein the Thr residue located between the fifth and sixth conserved cysteines of the folded EGF-like domains is modified. This observation is supported by the fact that the expression of Eogt1 in Drosophila rescued the cell-adhesion defect caused by Eogt downregulation. In HEK293T cells, Eogt1 expression promoted modification of Notch1 EGF repeats by O-GlcNAc, which was further modified, at least in part, by galactose to generate a novel O-linked-N-acetyllactosamine structure. These results suggest that Eogt1 encodes EGF domain O-GlcNAc transferase and that O-GlcNAcylation reaction in the secretory pathway is a fundamental biochemical process conserved through evolution.
|
| 巻・号 |
419(1)
|
| ページ |
14-9
|
| 公開日 |
2012-3-2
|
| DOI |
10.1016/j.bbrc.2012.01.098
|
| PII |
S0006-291X(12)00145-3
|
| PMID |
22310717
|
| MeSH |
Acetylglucosamine / metabolism*
Amino Acid Sequence
Animals
Drosophila melanogaster / enzymology
Evolution, Molecular
Gene Expression
HEK293 Cells
Humans
Mice
Molecular Sequence Data
N-Acetylglucosaminyltransferases / chemistry
N-Acetylglucosaminyltransferases / genetics
N-Acetylglucosaminyltransferases / metabolism*
Receptors, Notch / metabolism*
|
| IF |
2.985
|
| 引用数 |
61
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
9867R-1
9867R-3 |
