RRC ID 22038
Author Eddison M, Belay AT, Sokolowski MB, Heberlein U.
Title A genetic screen for olfactory habituation mutations in Drosophila: analysis of novel foraging alleles and an underlying neural circuit.
Journal PLoS One
Abstract Habituation is a form of non-associative learning that enables animals to reduce their reaction to repeated harmless stimuli. When exposed to ethanol vapor, Drosophila show an olfactory-mediated startle response characterized by a transient increase in locomotor activity. Upon repeated exposures, this olfactory startle attenuates with the characteristics of habituation. Here we describe the results of a genetic screen to identify olfactory startle habituation (OSH) mutants. One mutation is a transcript specific allele of foraging (for) encoding a cGMP-dependent kinase. We show this allele of for reduces expression of a for-T1 isoform expressed in the head and functions normally to inhibit OSH. We localize for-T1 function to a limited set of neurons that include olfactory receptor neurons (ORNs) and the mushroom body (MB). Overexpression of for-T1 in ORNs inhibits OSH, an effect also seen upon synaptic silencing of the ORNs; for-T1 may therefore function in ORNs to decrease synaptic release upon repeated exposure to ethanol vapor. Overall, this work contributes to our understanding of the genes and neurons underlying olfactory habituation in Drosophila.
Volume 7(12)
Pages e51684
Published 2012-12-17
DOI 10.1371/journal.pone.0051684
PII PONE-D-12-23013
Description PMC3524188
PMID 23284741
PMC PMC3524188
MeSH Alleles Animals Anti-Infective Agents, Local / toxicity Cyclic GMP-Dependent Protein Kinases / genetics* Cyclic GMP-Dependent Protein Kinases / metabolism Drosophila Proteins / genetics* Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Ethanol / toxicity Genetic Testing Habituation, Psychophysiologic / drug effects Habituation, Psychophysiologic / genetics* Mushroom Bodies / injuries Mushroom Bodies / physiology* Mutation / genetics* Neurons / cytology Neurons / physiology* Olfactory Receptor Neurons / cytology Olfactory Receptor Neurons / physiology* Reaction Time Reflex, Startle / drug effects Reflex, Startle / physiology* Smell / physiology
IF 2.74
Times Cited 7
WOS Category NEUROSCIENCES
Resource
Drosophila NP2614 (DGRC#104259)