| RRC ID |
2425
|
| 著者 |
Yoshikawa N, Nakamura K, Yamaguchi Y, Kagota S, Shinozuka K, Kunitomo M.
|
| タイトル |
Reinforcement of antitumor effect of Cordyceps sinensis by 2'-deoxycoformycin, an adenosine deaminase inhibitor.
|
| ジャーナル |
In Vivo
|
| Abstract |
In this study, an attempt was made to elucidate the combined effect of 2'-deoxycoformycin (DCF), an adenosine deaminase inhibitor, with a water extract of Cordyceps sinensis (WECS), on the growth curves of mouse melanoma and lung carcinoma cells. Sub-confluent cells were harvested with an EDTA trypsin solution, and resuspended to appropriate concentrations in DMEM containing 10% fetal bovine serum. Using 1x10(5) cells/2 ml in each well of a 12-well culture plate, cells were incubated for 24, 48 and 72 h in the presence of WECS alone, or WECS plus DCF in a CO2 incubator at 37 degrees C. Duplicate samples of viable cells were enumerated with a Coulter counter. The antitumor effect of WECS on the growth curves of tumor cell lines increased over 3-fold in combination with DCF. These results suggest that DCF has a remarkable reinforcement effect on the antitumor activity of WECS. DCF is a potent adjuvant for WECS.
|
| 巻・号 |
21(2)
|
| ページ |
291-5
|
| 公開日 |
2007-1-1
|
| PMID |
17436579
|
| MeSH |
Animals
Antibiotics, Antineoplastic / pharmacology
Antineoplastic Agents / pharmacology
Cell Division / drug effects*
Cell Line, Tumor
Complex Mixtures / therapeutic use*
Cordyceps*
Deoxyadenosines / pharmacology*
Kinetics
Medicine, Chinese Traditional
Melanoma
Mice
Pentostatin / pharmacology*
|
| IF |
1.541
|
| 引用数 |
8
|
| リソース情報 |
| ヒト・動物細胞 |
|
