Reference - Detail
RRC ID | 2457 |
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Author | Sato T, Takahoko M, Okamoto H. |
Title | HuC:Kaede, a useful tool to label neural morphologies in networks in vivo. |
Journal | Genesis |
Abstract |
A simple and efficient procedure for labeling neurons is a prerequisite for investigating the development of neural networks in zebrafish. To label neurons we used Kaede, a fluorescent protein with a photoconversion property allowing conversion from green to red fluorescence following irradiation with UV or violet light. We established a zebrafish stable transgenic line, Tg(HuC:Kaede), expressing Kaede in neurons under the control of the HuC promoter. This transgenic line was used to label a small number of neurons in the trigeminal ganglion. Also, using embryos injected with the transgene, we labeled peripheral axon arbors of a Rohon-Beard neuron at 4 days postfertilization and observed the dendrite development of a tectal neuron for 3 days. These data indicate that Kaede is a useful tool to selectively label neural networks in zebrafish. |
Volume | 44(3) |
Pages | 136-42 |
Published | 2006-3-1 |
DOI | 10.1002/gene.20196 |
PMID | 16496337 |
MeSH | Animals Dendrites / physiology ELAV Proteins / genetics* ELAV-Like Protein 3 Embryo, Nonmammalian / metabolism Gene Expression Regulation, Developmental / physiology* Luminescent Proteins / genetics* Microinjections Nerve Net / cytology Nerve Net / embryology* Neurons / cytology* Recombinant Fusion Proteins / metabolism Staining and Labeling / methods Transgenes Trigeminal Ganglion / cytology Trigeminal Ganglion / embryology* Zebrafish / embryology Zebrafish Proteins / genetics* |
IF | 1.76 |
Times Cited | 112 |
WOS Category | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY |
Resource | |
Zebrafish | rw0130aTg (Huc:Kaede) |