| RRC ID |
2510
|
| 著者 |
Shitsukawa N, Tahira C, Kassai K, Hirabayashi C, Shimizu T, Takumi S, Mochida K, Kawaura K, Ogihara Y, Murai K.
|
| タイトル |
Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat.
|
| ジャーナル |
Plant Cell
|
| Abstract |
Bread wheat (Triticum aestivum) is a hexaploid species with A, B, and D ancestral genomes. Most bread wheat genes are present in the genome as triplicated homoeologous genes (homoeologs) derived from the ancestral species. Here, we report that both genetic and epigenetic alterations have occurred in the homoeologs of a wheat class E MADS box gene. Two class E genes are identified in wheat, wheat SEPALLATA (WSEP) and wheat LEAFY HULL STERILE1 (WLHS1), which are homologs of Os MADS45 and Os MADS1 in rice (Oryza sativa), respectively. The three wheat homoeologs of WSEP showed similar genomic structures and expression profiles. By contrast, the three homoeologs of WLHS1 showed genetic and epigenetic alterations. The A genome WLHS1 homoeolog (WLHS1-A) had a structural alteration that contained a large novel sequence in place of the K domain sequence. A yeast two-hybrid analysis and a transgenic experiment indicated that the WLHS1-A protein had no apparent function. The B and D genome homoeologs, WLHS1-B and WLHS1-D, respectively, had an intact MADS box gene structure, but WLHS1-B was predominantly silenced by cytosine methylation. Consequently, of the three WLHS1 homoeologs, only WLHS1-D functions in hexaploid wheat. This is a situation where three homoeologs are differentially regulated by genetic and epigenetic mechanisms.
|
| 巻・号 |
19(6)
|
| ページ |
1723-37
|
| 公開日 |
2007-6-1
|
| DOI |
10.1105/tpc.107.051813
|
| PII |
tpc.107.051813
|
| PMID |
17586655
|
| PMC |
PMC1955732
|
| MeSH |
Arabidopsis / genetics
Base Sequence
DNA Methylation
Epigenesis, Genetic*
Flowers / metabolism
Gene Expression Regulation, Plant
Genes, Plant / genetics*
Hybridization, Genetic
MADS Domain Proteins / genetics*
Molecular Sequence Data
Phenotype
Phylogeny
Plant Proteins / genetics
Plant Proteins / metabolism
Plants, Genetically Modified
Polyploidy*
Promoter Regions, Genetic / genetics
Protein Binding
Sequence Homology, Nucleic Acid
Triticum / genetics*
|
| IF |
9.618
|
| 引用数 |
99
|
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| コムギ |
90 lines |
