| RRC ID |
2564
|
| Author |
Lau SCK, Tsoi MMY, Li X, Plakhotnikova I, Wu M, Wong PK, Qian PY.
|
| Title |
Loktanella hongkongensis sp. nov., a novel member of the alpha-Proteobacteria originating from marine biofilms in Hong Kong waters.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A Gram-negative, non-motile, non-spore-forming, short rod-shaped bacterium (UST950701-009P(T)) was isolated from a marine biofilm in Hong Kong waters. Colonies are pink in colour, convex with a smooth surface and entire edge. Brown diffusible pigment is produced. Whitish colonies, with otherwise identical morphology, emerge from every culture upon ageing. The white colonies can be maintained as separate cultures (UST950701-009W) without turning pink. UST950701-009P(T) and UST950701-009W have identical 16S rRNA gene sequences and similar G+C (65.9-66.2 mol%) and fatty acid (86.22-88.52 % 18 : 1omega7c) contents. Phylogenetic analysis of the 16S rRNA gene sequence places UST950701-009P(T) within the Rhodobacter group of the alpha-subclass of the Proteobacteria. The nearest neighbours belong to the genus Loktanella, with similarity values ranging from 94.5 to 95.5 %. Data on G+C and fatty acid contents support the affiliation to the genus Loktanella. UST950701-009P(T) and -009W are heterotrophic, strictly aerobic and require NaCl for growth (2.0-14.0 %). Both grow in pH 5.0-10.0 and at 8-44 degrees C. Both are positive in oxidase, catalase and beta-galactosidase tests, but they differ in the pattern of carbohydrate oxidation and assimilation. Molecular evidence together with phenotypic characteristics shows that UST950701-009P(T) constitutes a novel species within the genus Loktanella. The name Loktanella hongkongensis sp. nov. is proposed; the type strain is UST950701-009P(T) (=NRRL B-41039(T)=JCM 12479(T)) and a morphovar is UST950701-009W (=NRRL B-41040=JCM 12480).
|
| Volume |
54(Pt 6)
|
| Pages |
2281-2284
|
| Published |
2004-11-1
|
| DOI |
10.1099/ijs.0.63294-0
|
| PMID |
15545471
|
| MeSH |
Aerobiosis
Bacterial Typing Techniques
Base Composition
Biofilms*
Carbohydrate Metabolism
Catalase / analysis
DNA, Bacterial / chemistry
DNA, Bacterial / isolation & purification
DNA, Ribosomal / chemistry
DNA, Ribosomal / isolation & purification
Fatty Acids / analysis
Fatty Acids / isolation & purification
Genes, rRNA
Gentian Violet
Hong Kong
Hydrogen-Ion Concentration
Molecular Sequence Data
Movement
Oxidoreductases / analysis
Phenazines
Phylogeny
Pigments, Biological / biosynthesis
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Rhodobacteraceae / classification*
Rhodobacteraceae / cytology
Rhodobacteraceae / isolation & purification*
Rhodobacteraceae / physiology
Saline Solution, Hypertonic
Seawater / microbiology*
Sequence Analysis, DNA
Spores, Bacterial / cytology
Temperature
Water Microbiology
beta-Galactosidase / analysis
|
| IF |
2.415
|
| Times Cited |
42
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| Pathogenic microorganisms |
JCM 12479?
JCM 12480?
JCM 7684? |
