| RRC ID |
2769
|
| 著者 |
Iigaya K, Kumagai H, Onimaru H, Kawai A, Oshima N, Onami T, Takimoto C, Kamayachi T, Hayashi K, Saruta T, Itoh H.
|
| タイトル |
Novel axonal projection from the caudal end of the ventrolateral medulla to the intermediolateral cell column.
|
| ジャーナル |
Am J Physiol Regul Integr Comp Physiol
|
| Abstract |
We used an optical imaging technique to investigate whether axons of neurons in the caudal end of the ventrolateral medulla (CeVLM), as well as axons of neurons in the rostral ventrolateral medulla (RVLM), project to neurons in the intermediolateral cell column (IML) of the spinal cord. Brain stem-spinal cord preparations from neonatal normotensive Wistar-Kyoto and spontaneously hypertensive rats were stained with a voltage-sensitive dye, and responses to electrical stimulation of the IML at the Th2 level were detected as changes in fluorescence intensity with an optical imaging apparatus (MiCAM-01). The results were as follows: 1) depolarizing responses to IML stimulation during low-Ca high-Mg superfusion were detected on the ventral surface of the medulla at the level of the CeVLM, as well as at the level of the RVLM, 2) depolarizing responses were also detected on cross sections at the level of the CeVLM, and they had a latency of 24.0 +/- 5.5 (SD) ms, 3) antidromic action potentials in response to IML stimulation were demonstrated in the CeVLM neurons where optical images were detected, and 4) glutamate application to the CeVLM increased the frequency of excitatory postsynaptic potentials (EPSPs) and induced depolarization of the IML neurons. The optical imaging findings suggested a novel axonal and functional projection from neurons in the CeVLM to the IML. The increase in EPSPs of the IML neurons in response to glutamate application suggests that the CeVLM participates in the regulation of sympathetic nerve activity and blood pressure and may correspond to the caudal pressor area.
|
| 巻・号 |
292(2)
|
| ページ |
R927-36
|
| 公開日 |
2007-2-1
|
| DOI |
10.1152/ajpregu.00254.2006
|
| PII |
00254.2006
|
| PMID |
17082356
|
| MeSH |
Action Potentials / physiology
Animals
Animals, Newborn
Axons / physiology*
Brain Stem / cytology
Brain Stem / physiology
Calcium
Electric Stimulation
Electrophysiology
Excitatory Postsynaptic Potentials / drug effects
Glutamic Acid / pharmacology
Image Processing, Computer-Assisted
Magnesium
Medulla Oblongata / cytology
Medulla Oblongata / physiology*
Neural Pathways / cytology
Neural Pathways / physiology*
Rats
Rats, Inbred SHR
Rats, Inbred WKY
Spinal Cord / cytology
Spinal Cord / physiology
gamma-Aminobutyric Acid / physiology
|
| IF |
3.026
|
| 引用数 |
12
|
|
WOS 分野
|
PHYSIOLOGY
|
| リソース情報 |
| ラット |
|
