論文 - 詳細
| RRC ID | 28105 |
|---|---|
| 著者 | Hamasaki H, Yoshizumi T, Takahashi N, Higuchi M, Kuromori T, Imura Y, Shimada H, Matsui M. |
| タイトル | SD3, an Arabidopsis thaliana homolog of TIM21, affects intracellular ATP levels and seedling development. |
| ジャーナル | Mol Plant |
| Abstract |
It is poorly understood how plants control their growth by cell division, elongation, and differentiation. We have characterized a seedling-lethal mutant segregation distortion 3 (sd3) that showed a very dwarf phenotype when grown in the light and, in the dark, had short hypocotyls with reduced ploidy levels. The corresponding gene of SD3 encodes a protein with high similarity to yeast translocase on the inner mitochondrial membrane 21 (TIM21), which is a component of the TIM23 complex. Indeed, SD3 protein fused to GFP localized in the mitochondria. SD3 overexpression increased cotyledon size in the light and hypocotyl thickness in the dark. The expression of genes for several subunits of the respiratory-chain complexes III and IV was up-regulated in SD3-overexpressing plants. Furthermore, these plants showed high levels of ATP whereas those of sd3 were low. These results suggested that SD3 induced an increase in cell size by raising the expression of the respiratory-chain subunit genes and hence increased the intracellular ATP levels. We propose that intracellular ATP levels regulated by mitochondria control plant organ size. |
| 巻・号 | 5(2) |
| ページ | 461-71 |
| 公開日 | 2012-3-1 |
| DOI | 10.1093/mp/ssr088 |
| PII | S1674-2052(14)60133-7 |
| PMID | 22131050 |
| MeSH | Adenosine Triphosphate / metabolism* Amino Acid Sequence Arabidopsis / cytology Arabidopsis / genetics Arabidopsis / growth & development* Arabidopsis / radiation effects Arabidopsis Proteins / chemistry Arabidopsis Proteins / metabolism* Cell Count Cell Size Gene Expression Regulation, Plant / radiation effects Hypocotyl / growth & development Hypocotyl / radiation effects Intracellular Space / metabolism* Intracellular Space / radiation effects Light Mitochondrial Membrane Transport Proteins / chemistry* Mitochondrial Membranes / metabolism Mitochondrial Membranes / radiation effects Mitochondrial Precursor Protein Import Complex Proteins Mitochondrial Proteins / chemistry Mitochondrial Proteins / metabolism* Molecular Sequence Data Mutation / genetics Phenotype Polyploidy Saccharomyces cerevisiae Proteins / chemistry* Seedlings / genetics Seedlings / growth & development* Seedlings / radiation effects Sequence Homology, Amino Acid* Up-Regulation / radiation effects |
| IF | 12.084 |
| 引用数 | 18 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst15182 |