Reference - Detail
| RRC ID | 29091 |
|---|---|
| Author | Riedl J, Crevenna AH, Kessenbrock K, Yu JH, Neukirchen D, Bista M, Bradke F, Jenne D, Holak TA, Werb Z, Sixt M, Wedlich-Soldner R. |
| Title | Lifeact: a versatile marker to visualize F-actin. |
| Journal | Nat Methods |
| Abstract |
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells. |
| Volume | 5(7) |
| Pages | 605-7 |
| Published | 2008-7-1 |
| DOI | 10.1038/nmeth.1220 |
| PII | nmeth.1220 |
| PMID | 18536722 |
| PMC | PMC2814344 |
| MeSH | Actins / metabolism* Animals Cells, Cultured Coloring Agents / chemistry* Cytoskeleton / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Humans Mice Microscopy, Fluorescence Peptides / chemistry* Rats Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Staining and Labeling / methods* |
| IF | 30.822 |
| Times Cited | 1213 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS |
| Resource | |
| Cellular slime molds | S00444 G90096 |