Reference - Detail
| RRC ID | 29126 |
|---|---|
| Author | Yahara N, Ueda T, Sato K, Nakano A. |
| Title | Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways. |
| Journal | Mol Biol Cell |
| Abstract |
ADP-ribosylation factors, a family of small GTPases, are believed to be key regulators of intracellular membrane traffic. However, many biochemical in vitro experiments have led to different models for their involvement in various steps of vesicular transport, and their precise role in living cells is still unclear. We have taken advantage of the powerful yeast genetic system and screened for temperature-sensitive (ts) mutants of the ARF1 gene from Saccharomyces cerevisiae. By random mutagenesis of the whole open reading frame of ARF1 by error-prone PCR, we isolated eight mutants and examined their phenotypes. arf1 ts mutants showed a variety of transport defects and morphological alterations in an allele-specific manner. Furthermore, intragenic complementation was observed between certain pairs of mutant alleles, both for cell growth and intracellular transport. These results demonstrate that the single Arf1 protein is indeed involved in many different steps of intracellular transport in vivo and that its multiple roles may be dissected by the mutant alleles we constructed. |
| Volume | 12(1) |
| Pages | 221-38 |
| Published | 2001-1-1 |
| DOI | 10.1091/mbc.12.1.221 |
| PMID | 11160834 |
| PMC | PMC30579 |
| MeSH | ADP-Ribosylation Factor 1 / genetics ADP-Ribosylation Factor 1 / metabolism* ADP-Ribosylation Factor 1 / physiology Amino Acid Sequence Biological Transport / drug effects Endocytosis / drug effects* Exocytosis / drug effects* Microscopy, Electron Molecular Sequence Data Mutagenesis, Site-Directed Mutation Phenotype Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / physiology Sequence Alignment Transport Vesicles / drug effects Yeasts / cytology Yeasts / physiology* |
| IF | 3.791 |
| Times Cited | 55 |
| WOS Category | CELL BIOLOGY |
| Resource | |
| DNA material | arf1 delta (RDB08577) arf2 delta (RDB08578) pNY14-A1S (RDB08579) pNY16-A1 (RDB08736) pNY605 (RDB08737) pNY606NB (RDB08741) pNY607NB (RDB08738) pNY801 (RDB08739) pNY902 (RDB08931) pNY902NB (RDB08743) pNY1004 (RDB08740) pNY1005CB (RDB08744) 605::ADE2 (RDB08745) 607NB::ADE2 (RDB08746) 801::ADE2 (RDB08747) 606NB::ADE2 (RDB08748) 902::ADE2 (RDB08749) 902NB::ADE2 (RDB08750) 1005CB::ADE2 (RDB08751) pOTA1-1::ADE2 (YIpARF1) (RDB08576) |