RRC ID |
29410
|
著者 |
Yang G, Chen M, Zhou S, Liu Z, Yuan Y.
|
タイトル |
Sinorhodobacter ferrireducens gen. nov., sp. nov., a non-phototrophic iron-reducing bacterium closely related to phototrophic Rhodobacter species.
|
ジャーナル |
Antonie Van Leeuwenhoek
|
Abstract |
An Fe(III) oxide-reducing bacterium designated as SgZ-3(T), which could couple glucose oxidation to Fe(III) oxide reduction for energy conservation, was isolated from an electrochemical biofilm. The isolate was Gram-negative, catalase-positive and oxidase-negative, and facultative anaerobic. NaCl was not required for growth, but NaCl concentrations up to 5 % (w/v) were tolerated. Growth occurred in TSB (tryptic soy broth) at 20-40 °C (optimum 30 °C) and at pH 6.0-7.5 (optimum 7.0). Phototrophic growth could not be demonstrated. No vesicular photosynthetic membrane was observed. Bacteriochlorophyll α and carotenoids were absent. Biotin and thiamine were required as growth factors for the isolate. Phylogenetic analysis of the 16S rRNA gene sequence placed strain SgZ-3(T) within the family Rhodobacteraceae and affiliated with an phototrophic genera Rhodobacter. The G+C content of the genomic DNA was 68.6 mol%. Strain SgZ-3(T) contained Q-10 as the predominant quinone. The major cellular fatty acids were C18:1 ω6c and/or C18:1 ω7c (66.9 %) and C16:0 (9.5 %). Based on its phenotypic and phylogenetic properties, chemotaxonomic analysis and the results of physiological and biochemical tests, strain SgZ-3(T) (=KACC 16603(T) = CCTCC AB2012026(T)) was designated as the type strain of a new genus and a novel species of the family Rhodobacteraceae, for which the name Sinorhodobacter ferrireducens sp. nov. was proposed.
|
巻・号 |
104(5)
|
ページ |
715-24
|
公開日 |
2013-11-1
|
DOI |
10.1007/s10482-013-9979-0
|
PMID |
23907520
|
MeSH |
Bacterial Typing Techniques
Base Composition
Catalase / metabolism
Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Environmental Microbiology
Fatty Acids / analysis
Glucose / metabolism
Hydrogen-Ion Concentration
Iron / metabolism*
Molecular Sequence Data
Oxidation-Reduction
Oxidoreductases / metabolism
Phototrophic Processes
Phylogeny
Quinones / analysis
RNA, Ribosomal, 16S / genetics
Rhodobacteraceae / classification*
Rhodobacteraceae / genetics
Rhodobacteraceae / isolation & purification*
Sequence Analysis, DNA
Sodium Chloride / metabolism
Temperature
|
IF |
2.674
|
引用数 |
7
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 14794
JCM 14887 |