| RRC ID |
29780
|
| Author |
Timmis K, Cabello F, Cohen SN.
|
| Title |
Cloning, isolation, and characterization of replication regions of complex plasmid genomes.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
EcoRI endonuclease-generated DNA fragments carrying replication regions of the F'lac and R6-5 plasmids have been cloned and isolated, using as a selection vehicle a nonreplicating ampicillin-resistance DNA fragment derived from a Staphylococcus aureus plasmid. Heteroduplex analysis of the constructed plasmid chimeras and the parent replicons has localized the cloned R6-5 replication region to a DNA segment between kilobase pair coordinates 1.0 and 88.0 on the R6-5 map. Physical proximity between the plasmid replication functions and the locus governing plasmid incompatibility has been shown for both parent replicons. The cloning method reported appears to be generally applicable for the identification and isolation of replication regions of a variety of complex genomes.
|
| Volume |
72(6)
|
| Pages |
2242-6
|
| Published |
1975-6-1
|
| DOI |
10.1073/pnas.72.6.2242
|
| PMID |
1094460
|
| PMC |
PMC432733
|
| MeSH |
Centrifugation, Density Gradient
DNA Replication*
DNA Restriction Enzymes
DNA, Bacterial / isolation & purification*
Electrophoresis, Agar Gel
Escherichia coli / analysis*
Escherichia coli / ultrastructure
Extrachromosomal Inheritance*
Genetics, Microbial
Hybridization, Genetic
Microscopy, Electron
Molecular Weight
Phenotype
Plasmids*
Selection, Genetic
Staphylococcus
Transformation, Genetic
|
| IF |
9.412
|
| Resource |
| Prokaryotes E. coli |
ME6171
ME6172
ME6163 |
