論文 - 詳細
RRC ID | 30071 |
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著者 | Burns DM, Beacham IR. |
タイトル | Positive selection vectors: a small plasmid vector useful for the direct selection of Sau3A-generated overlapping DNA fragments. |
ジャーナル | Gene |
Abstract |
A positive selection plasmid vector ( pLA7 ), containing a unique BclI site, and its use in the facile selection of a library of overlapping DNA fragments, generated by a partial digest with Sau3A, is described. Selection depends on the 5-fluorouracil + 5' AMP resistance of upp- ush - [ pLA7ush ::cloned DNA] cells, whereas upp- ush - [ pLA7ush +] cells are sensitive under the same conditions. |
巻・号 | 27(3) |
ページ | 323-5 |
公開日 | 1984-3-1 |
DOI | 10.1016/0378-1119(84)90077-5 |
PII | 0378-1119(84)90077-5 |
PMID | 6329908 |
MeSH | Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes* Deoxyribonucleases, Type II Site-Specific* Genetic Vectors* Nucleotidases / genetics Plasmids* Selection, Genetic |
IF | 2.984 |
引用数 | 18 |
WOS 分野 | GENETICS & HEREDITY |
リソース情報 | |
原核生物(大腸菌) | ME8614 pLA7 |