RRC ID |
30195
|
著者 |
Mizuno K, Shiba K, Okai M, Takahashi Y, Shitaka Y, Oiwa K, Tanokura M, Inaba K.
|
タイトル |
Calaxin drives sperm chemotaxis by Ca²⁺-mediated direct modulation of a dynein motor.
|
ジャーナル |
Proc Natl Acad Sci U S A
|
Abstract |
Sperm chemotaxis occurs widely in animals and plants and plays an important role in the success of fertilization. Several studies have recently demonstrated that Ca(2+) influx through specific Ca(2+) channels is a prerequisite for sperm chemotactic movement. However, the regulator that modulates flagellar movement in response to Ca(2+) is unknown. Here we show that a neuronal calcium sensor, calaxin, directly acts on outer-arm dynein and regulates specific flagellar movement during sperm chemotaxis. Calaxin inhibition resulted in significant loss of sperm chemotactic movement, despite normal increases in intracellular calcium concentration. Using a demembranated sperm model, we demonstrate that calaxin is essential for generation and propagation of Ca(2+)-induced asymmetric flagellar bending. An in vitro motility assay revealed that calaxin directly suppressed the velocity of microtubule sliding by outer-arm dynein at high Ca(2+) concentrations. This study describes the missing link between chemoattractant-mediated Ca(2+) signaling and motor-driven microtubule sliding during sperm chemotaxis.
|
巻・号 |
109(50)
|
ページ |
20497-502
|
公開日 |
2012-12-11
|
DOI |
10.1073/pnas.1217018109
|
PII |
1217018109
|
PMID |
23169663
|
PMC |
PMC3528578
|
MeSH |
Animals
Calcium Signaling / physiology
Carbamates / pharmacology
Chemotaxis / drug effects
Chemotaxis / physiology
Ciona intestinalis / cytology
Ciona intestinalis / physiology
Dyneins / physiology*
Intracellular Calcium-Sensing Proteins / physiology*
Male
Microtubules / physiology
Models, Biological
Molecular Motor Proteins / physiology
Piperidines / pharmacology
Sperm Motility / physiology
Sperm Tail / physiology
Spermatozoa / drug effects
Spermatozoa / physiology*
|
IF |
9.412
|
引用数 |
41
|
WOS 分野
|
CELL BIOLOGY
|
リソース情報 |
カタユウレイボヤ・(ニッポンウミシダ) |
Wild C. int |