Reference - Detail
| RRC ID | 30641 |
|---|---|
| Author | Cheerambathur DK, Gassmann R, Cook B, Oegema K, Desai A. |
| Title | Crosstalk between microtubule attachment complexes ensures accurate chromosome segregation. |
| Journal | Science |
| Abstract |
The microtubule-based mitotic spindle segregates chromosomes during cell division. During chromosome segregation, the centromeric regions of chromosomes build kinetochores that establish end-coupled attachments to spindle microtubules. Here, we used the Caenorhabditis elegans embryo as a model system to examine the crosstalk between two kinetochore protein complexes implicated in temporally distinct stages of attachment formation. The kinetochore dynein module, which mediates initial lateral microtubule capture, inhibited microtubule binding by the Ndc80 complex, which ultimately forms the end-coupled attachments that segregate chromosomes. The kinetochore dynein module directly regulated Ndc80, independently of phosphorylation by Aurora B kinase, and this regulation was required for accurate segregation. Thus, the conversion from initial dynein-mediated, lateral attachments to correctly oriented, Ndc80-mediated end-coupled attachments is actively controlled. |
| Volume | 342(6163) |
| Pages | 1239-42 |
| Published | 2013-12-6 |
| DOI | 10.1126/science.1246232 |
| PII | science.1246232 |
| PMID | 24231804 |
| PMC | PMC3885540 |
| MeSH | Amino Acid Motifs Animals Aurora Kinase B / metabolism Caenorhabditis elegans / embryology Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / chemistry Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Chromosome Segregation* Dyneins / metabolism* Embryo, Nonmammalian / metabolism Kinetochores / metabolism* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Microtubules / metabolism* Multiprotein Complexes / metabolism Phenotype Phosphorylation Protein Binding Spindle Apparatus / metabolism* Transgenes |
| IF | 41.846 |
| Times Cited | 48 |
| WOS Category | CELL BIOLOGY |
| Resource | |
| C.elegans | tm5271 |