RRC ID |
30675
|
著者 |
Gottar M, Gobert V, Michel T, Belvin M, Duyk G, Hoffmann JA, Ferrandon D, Royet J.
|
タイトル |
The Drosophila immune response against Gram-negative bacteria is mediated by a peptidoglycan recognition protein.
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ジャーナル |
Nature
|
Abstract |
The antimicrobial defence of Drosophila relies largely on the challenge-induced synthesis of an array of potent antimicrobial peptides by the fat body. The defence against Gram-positive bacteria and natural fungal infections is mediated by the Toll signalling pathway, whereas defence against Gram-negative bacteria is dependent on the Immune deficiency (IMD) pathway. Loss-of-function mutations in either pathway reduce the resistance to corresponding infections. The link between microbial infections and activation of these two pathways has remained elusive. The Toll pathway is activated by Gram-positive bacteria through a circulating Peptidoglycan recognition protein (PGRP-SA). PGRPs appear to be highly conserved from insects to mammals, and the Drosophila genome contains 13 members. Here we report a mutation in a gene coding for a putative transmembrane protein, PGRP-LC, which reduces survival to Gram-negative sepsis but has no effect on the response to Gram-positive bacteria or natural fungal infections. By genetic epistasis, we demonstrate that PGRP-LC acts upstream of the imd gene. The data on PGRP-SA with respect to the response to Gram-positive infections, together with the present report, indicate that the PGRP family has a principal role in sensing microbial infections in Drosophila.
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巻・号 |
416(6881)
|
ページ |
640-4
|
公開日 |
2002-4-11
|
DOI |
10.1038/nature734
|
PII |
nature734
|
PMID |
11912488
|
MeSH |
Animals
Anti-Bacterial Agents / metabolism
Carrier Proteins / biosynthesis
Carrier Proteins / genetics
Carrier Proteins / immunology*
Drosophila Proteins / genetics
Drosophila Proteins / metabolism
Drosophila melanogaster / genetics
Drosophila melanogaster / immunology*
Drosophila melanogaster / microbiology*
Epistasis, Genetic
Female
Genes, Insect / genetics
Genetic Predisposition to Disease
Gram-Negative Bacteria / immunology*
Gram-Negative Bacteria / physiology
Humans
Mutation
Phenotype
RNA, Messenger / genetics
RNA, Messenger / metabolism
Signal Transduction
Survival Rate
Transgenes / genetics
|
IF |
42.779
|
引用数 |
453
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ショウジョウバエ |
|