| RRC ID |
30729
|
| 著者 |
Kobayashi S, Tsugama D, Liu S, Takano T.
|
| タイトル |
A U-Box E3 ubiquitin ligase, PUB20, interacts with the Arabidopsis G-protein β subunit, AGB1.
|
| ジャーナル |
PLoS One
|
| Abstract |
An Arabidopsis U-box E3 ubiquitin ligase Plant U-box 20 (PUB20; alternatively called AtCMPG1) was identified as a possible interactor of the Arabidopsis G-protein β subunit, AGB1, by yeast two-hybrid screening. A bimolecular fluorescence complementation (BiFC) assay showed that PUB20 interacted with AGB1 in the nuclei and the cytosol. The expression levels of PUB20 and its closest homolog, PUB21 were stable under many conditions. GUS driven by the PUB20 promoter was active in anthers, pollen, premature seeds and receptacles and GUS driven by the PUB21 promoter was active in anthers and funiculi. PUB20 was found to have autoubiquitination activity in vitro.
|
| 巻・号 |
7(11)
|
| ページ |
e49207
|
| 公開日 |
2012-1-1
|
| DOI |
10.1371/journal.pone.0049207
|
| PII |
PONE-D-12-18615
|
| PMID |
23166612
|
| PMC |
PMC3499536
|
| MeSH |
Arabidopsis / metabolism*
Arabidopsis Proteins / metabolism*
DNA Primers / genetics
GTP-Binding Protein beta Subunits / metabolism*
Promoter Regions, Genetic / genetics
Reverse Transcriptase Polymerase Chain Reaction
Two-Hybrid System Techniques
Ubiquitin-Protein Ligases / metabolism*
Ubiquitination
|
| IF |
2.74
|
| 引用数 |
12
|
|
WOS 分野
|
PLANT SCIENCES
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda01364 |
