Reference - Detail
| RRC ID | 30945 |
|---|---|
| Author | Parent CA, Blacklock BJ, Froehlich WM, Murphy DB, Devreotes PN. |
| Title | G protein signaling events are activated at the leading edge of chemotactic cells. |
| Journal | Cell |
| Abstract |
Directional sensing by eukaryotic cells does not require polarization of chemoattractant receptors. The translocation of the PH domain-containing protein CRAC in D. discoideum to binding sites on the inner face of the plasma membrane reflects activation of the G protein-linked signaling system. Increments in chemoattractant elicit a uniform response around the cell periphery. Yet when cells are exposed to a gradient, the activation occurs selectively at the stimulated edge, even in immobilized cells. We propose that such localized activation, transmitted by the recruitment of cytosolic proteins, may be a general mechanism for gradient sensing by G protein-linked chemotactic systems including those involving chemotactic cytokines in leukocytes. |
| Volume | 95(1) |
| Pages | 81-91 |
| Published | 1998-10-2 |
| DOI | 10.1016/s0092-8674(00)81784-5 |
| PII | S0092-8674(00)81784-5 |
| PMID | 9778249 |
| MeSH | Animals Binding Sites Biological Transport Cell Fractionation Cell Membrane / metabolism Chemotactic Factors / pharmacology Chemotaxis* Cyclic AMP / pharmacology Cytosol / metabolism Dictyostelium GTP-Binding Proteins / metabolism* Green Fluorescent Proteins Hydrogen-Ion Concentration Luminescent Proteins / genetics Luminescent Proteins / metabolism Protozoan Proteins / genetics Protozoan Proteins / metabolism* Signal Transduction* |
| IF | 38.637 |
| Times Cited | 462 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Cellular slime molds | G90001 G90004 |