論文 - 詳細
| RRC ID | 3115 |
|---|---|
| 著者 | Walker KL, Müller S, Moss D, Ehrhardt DW, Smith LG. |
| タイトル | Arabidopsis TANGLED identifies the division plane throughout mitosis and cytokinesis. |
| ジャーナル | Curr Biol |
| Abstract |
BACKGROUND:In premitotic plant cells, the future division plane is predicted by a cortical ring of microtubules and F-actin called the preprophase band (PPB). The PPB persists throughout prophase, but is disassembled upon nuclear-envelope breakdown as the mitotic spindle forms. Following nuclear division, a cytokinetic phragmoplast forms between the daughter nuclei and expands laterally to attach the new cell wall at the former PPB site. A variety of observations suggest that expanding phragmoplasts are actively guided to the former PPB site, but little is known about how plant cells "remember" this site after PPB disassembly. RESULTS:In premitotic plant cells, Arabidopsis TANGLED fused to YFP (AtTAN::YFP) colocalizes at the future division plane with PPBs. Strikingly, cortical AtTAN::YFP rings persist after PPB disassembly, marking the division plane throughout mitosis and cytokinesis. The AtTAN::YFP ring is relatively broad during preprophase/prophase and mitosis; narrows to become a sharper, more punctate ring during cytokinesis; and then rapidly disassembles upon completion of cytokinesis. The initial recruitment of AtTAN::YFP to the division plane requires microtubules and the kinesins POK1 and POK2, but subsequent maintenance of AtTAN::YFP rings appears to be microtubule independent. Consistent with the localization data, analysis of Arabidopsis tan mutants shows that AtTAN plays a role in guidance of expanding phragmoplasts to the former PPB site. CONCLUSIONS:AtTAN is implicated as a component of a cortical guidance cue that remains behind when the PPB is disassembled and directs the expanding phragmoplast to the former PPB site during cytokinesis. |
| 巻・号 | 17(21) |
| ページ | 1827-36 |
| 公開日 | 2007-11-6 |
| DOI | 10.1016/j.cub.2007.09.063 |
| PII | S0960-9822(07)02078-7 |
| PMID | 17964159 |
| PMC | PMC2134921 |
| MeSH | Arabidopsis / cytology Arabidopsis / metabolism* Arabidopsis Proteins / analysis Arabidopsis Proteins / metabolism* Cell Cycle Proteins / analysis Cell Cycle Proteins / metabolism* Cytokinesis* Kinesins / metabolism Meristem / cytology Meristem / metabolism Microtubules / chemistry Mitosis* Molecular Sequence Data Plants, Genetically Modified Recombinant Fusion Proteins / analysis Recombinant Fusion Proteins / metabolism |
| IF | 9.601 |
| 引用数 | 106 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst14955 |