論文 - 詳細
| RRC ID | 3208 |
|---|---|
| 著者 | Kobayashi N, Nishino K, Hirata T, Yamaguchi A. |
| タイトル | Membrane topology of ABC-type macrolide antibiotic exporter MacB in Escherichia coli. |
| ジャーナル | FEBS Lett |
| Abstract |
MacB is an ABC-type membrane protein that exports only macrolide compounds containing 14- and 15-membered lactones, cooperating with a membrane fusion protein, MacA, and a multifunctional outer membrane channel, TolC. We determined the membrane topology of MacB by means of site-specific competitive chemical modification of single cysteine mutants. As a result, it was revealed that MacB is composed of four transmembrane (TM) segments with a cytoplasmic N-terminal nucleotide binding domain of about 270 amino acid residues and a periplasmic large hydrophilic polypeptide between TM segments 1 and 2 of about 200 amino acid residues. |
| 巻・号 | 546(2-3) |
| ページ | 241-6 |
| 公開日 | 2003-7-10 |
| DOI | 10.1016/s0014-5793(03)00579-9 |
| PII | S0014579303005799 |
| PMID | 12832048 |
| MeSH | ATP-Binding Cassette Transporters / chemistry* ATP-Binding Cassette Transporters / genetics ATP-Binding Cassette Transporters / metabolism Amino Acid Sequence Carbon Radioisotopes Cell Membrane / chemistry Escherichia coli / chemistry* Escherichia coli / genetics Escherichia coli Proteins* Ethylmaleimide / metabolism Molecular Sequence Data Radioligand Assay |
| IF | 3.057 |
| 引用数 | 54 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | pBAD24 pBAD33 |