RRC ID |
33423
|
著者 |
Remaut E, Tsao H, Fiers W.
|
タイトル |
Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication.
|
ジャーナル |
Gene
|
Abstract |
Improved expression vectors have been constructed which are derived from runaway-replication mutants of plasmid R1 and carry the strong leftward promoter (pL) of bacteriophage lambda. The activity of this promoter is controlled by a temperature-sensitive repressor, product of the phage gene cI cloned on a compatible plasmid. Heat induction leads to amplification of the plasmid copy number and at the same time turns on the promoter. At a short distance downstream from the promoter, unique EcoRI, BamHI, XbaI and HindIII sites are present. This system was used for high level expression of the T4 DNA-ligase gene; 3 h after induction the ligase amounted to about 20% of total cellular protein.
|
巻・号 |
22(1)
|
ページ |
103-13
|
公開日 |
1983-4-1
|
DOI |
10.1016/0378-1119(83)90069-0
|
PII |
0378-1119(83)90069-0
|
PMID |
6305768
|
MeSH |
Bacteriophage lambda / genetics*
Escherichia coli / genetics*
Gene Amplification
Genetic Vectors*
Mutation
Operon
Plasmids*
Replicon
Species Specificity
Temperature
|
IF |
2.984
|
引用数 |
340
|
WOS 分野
|
GENETICS & HEREDITY
|
リソース情報 |
原核生物(大腸菌) |
pCP3
pCP40 |