Reference - Detail
RRC ID | 33551 |
---|---|
Author | Satomura T, Shimura D, Asai K, Sadaie Y, Hirooka K, Fujita Y. |
Title | Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system. |
Journal | J Bacteriol |
Abstract |
During DNA microarray analysis, we discovered that the GlnK-GlnL (formerly YcbA-YcbB) two-component system positively regulates the expression of the glsA-glnT (formerly ybgJ-ybgH) operon in response to glutamine in the culture medium on Northern analysis. As a result of gel retardation and DNase I footprinting analyses, we found that the GlnL protein interacts with a region (bases -13 to -56; +1 is the transcription initiation base determined on primer extension analysis of glsA-glnT) in which a direct repeat, TTTTGTN4TTTTGT, is present. Furthermore, the glsA and glnT genes were biochemically verified to encode glutaminase and glutamine transporter, respectively. |
Volume | 187(14) |
Pages | 4813-21 |
Published | 2005-7-1 |
DOI | 10.1128/JB.187.14.4813-4821.2005 |
PII | 187/14/4813 |
PMID | 15995196 |
PMC | PMC1169493 |
MeSH | Bacillus subtilis / genetics Bacillus subtilis / metabolism* Bacterial Proteins / metabolism Base Sequence Carrier Proteins / genetics Carrier Proteins / metabolism* DNA Primers Genes, Reporter Genotype Glutaminase / genetics Glutaminase / metabolism* Glutamine / metabolism* Histidine Kinase Kinetics Molecular Sequence Data Protein Kinases / genetics Protein Kinases / metabolism* Trans-Activators / genetics Trans-Activators / metabolism* beta-Galactosidase / genetics beta-Galactosidase / metabolism |
IF | 3.006 |
Times Cited | 30 |
WOS Category | MICROBIOLOGY |
Resource | |
Prokaryotes B. subtilis | MBS270 MBS271 MBS294 |