RRC ID |
33608
|
著者 |
Takeuchi M, Matsuda K, Yamaguchi S, Asakawa K, Miyasaka N, Lal P, Yoshihara Y, Koga A, Kawakami K, Shimizu T, Hibi M.
|
タイトル |
Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry.
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ジャーナル |
Dev Biol
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Abstract |
The cerebellum is involved in some forms of motor coordination and motor learning. Here we isolated transgenic (Tg) zebrafish lines that express a modified version of Gal4-VP16 (GFF) in the cerebellar neural circuits: granule, Purkinje, or eurydendroid cells, Bergmann glia, or the neurons in the inferior olive nuclei (IO) which send climbing fibers to Purkinje cells, with the transposon Tol2 system. By combining GFF lines with Tg lines carrying a reporter gene located downstream of Gal4 binding sequences (upstream activating sequence: UAS), we investigated the anatomy and developmental processes of the cerebellar neural circuitry. Combining an IO-specific Gal4 line with a UAS reporter line expressing the photoconvertible fluorescent protein Kaede demonstrated the contralateral projections of climbing fibers. Combining a granule cell-specific Gal4 line with a UAS reporter line expressing wheat germ agglutinin (WGA) confirmed direct and/or indirect connections of granule cells with Purkinje cells, eurydendroid cells, and IO neurons in zebrafish. Time-lapse analysis of a granule cell-specific Gal4 line revealed initial random movements and ventral migration of granule cell nuclei. Transgenesis of a reporter gene with another transposon Tol1 system visualized neuronal structure at a single cell resolution. Our findings indicate the usefulness of these zebrafish Gal4 Tg lines for studying the development and function of cerebellar neural circuits.
|
巻・号 |
397(1)
|
ページ |
1-17
|
公開日 |
2015-1-1
|
DOI |
10.1016/j.ydbio.2014.09.030
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PII |
S0012-1606(14)00499-0
|
PMID |
25300581
|
MeSH |
Animals
Animals, Genetically Modified
Cell Movement
Cerebellum / embryology*
Cerebellum / physiology
DNA Transposable Elements
DNA-Binding Proteins / genetics*
Enhancer Elements, Genetic
Gene Expression Regulation, Developmental
Genes, Reporter
Genetic Techniques
Green Fluorescent Proteins / metabolism
Neural Pathways
Neurons / physiology
Purkinje Cells / cytology
Synapses
Transcription Factors / genetics*
Transgenes
Zebrafish / embryology
Zebrafish / genetics
Zebrafish Proteins / genetics*
|
IF |
2.896
|
引用数 |
32
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
リソース情報 |
ゼブラフィッシュ |
rw0318(Tg(UAS:AcGFP-P2A-WGA))
gSAG6A
SAGFF(LF)128A
hspGFF57A
SAGFF(LF)157B
hspGFFDMC90A
hspzGFFgDMC156A
hspGFFDMC28C
SAGFF(LF)251A
SAGFF(LF)226B
nub6Tg(Tg(alloca:GFF))
rk22Tg(Tg(aldoca:gap43-Venus))
nub5Tg(Tg(aldoca:gap43-mCherry)) |