RRC ID |
34245
|
著者 |
Kobayashi Y, Hayashi M, Yoshino F, Tamura M, Yoshida A, Ibi H, Lee MC, Ochiai K, Ogiso B.
|
タイトル |
Passive ultrasonic irrigation in the presence of a low concentration of hydrogen peroxide enhances hydroxyl radical generation and bactericidal effect against Enterococcus faecalis.
|
ジャーナル |
J Oral Sci
|
Abstract |
Chemomechanical procedures can be used to eliminate bacteria from root canals. However, detectable bacteria sometimes remain because of the complexity of the root canal system. Endodontic passive ultrasonic irrigation (PUI) with hydrogen peroxide (H2O2) may be a promising option for increasing bactericidal hydroxyl radical (HO•) generation. In this in vitro experiment, we examined the effects of HO• generated using PUI and a low concentration of H2O2. An ultrasonic tip was submerged in 0.45 mol/L (1.5%) H2O2 in a microfuge tube. H2O2 was activated by an ultrasonic unit, the tip of which was kept centered in the tube, to mimic PUI. HO• generation was detected by electron spin resonance spectroscopy. An Enterococcus faecalis suspension in H2O2 was then preparedand activated as described above. Bactericidal effects were assessed by viable counting. Two-way analysis of variance and Tukey's test were used to assess the statistical significance of differences among groups (P < 0.05). HO• generation and bactericidal activity were significantly increased by PUI in H2O2 in a time-dependent manner and were significantly higher than with H2O2 alone or with PUI in a Tris-HCl suspension. These results suggest that PUI in the presence of a low H2O2 concentration is a promising new disinfection strategy.
|
巻・号 |
56(1)
|
ページ |
35-9
|
公開日 |
2014-3-1
|
DOI |
10.2334/josnusd.56.35
|
PII |
DN/JST.JSTAGE/josnusd/56.35
|
PMID |
24739706
|
MeSH |
Dose-Response Relationship, Drug
Enterococcus faecalis / metabolism*
Hydrogen Peroxide / pharmacology*
Hydroxyl Radical / metabolism*
Ultrasonics*
|
IF |
1.2
|
引用数 |
8
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
MATERIALS SCIENCE, BIOMATERIALS
|
リソース情報 |
一般微生物 |
JCM 5803 |