| RRC ID |
34304
|
| 著者 |
Modesto M, Michelini S, Stefanini I, Ferrara A, Tacconi S, Biavati B, Mattarelli P.
|
| タイトル |
Bifidobacterium aesculapii sp. nov., from the faeces of the baby common marmoset (Callithrix jacchus).
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Six Gram-positive-staining, microaerophilic, non-spore-forming, fructose-6-phosphate phosphoketolase-positive bacterial strains with a peculiar morphology were isolated from faecal samples of baby common marmosets (Callithrix jacchus). Cells of these strains showed a morphology not reported previously for a bifidobacterial species, which resembled a coiled snake, always coiled or ring shaped or forming a 'Y' shape. Strains MRM 3/1(T) and MRM 4/2 were chosen as representative strains and characterized further. The bacteria utilized a wide range of carbohydrates and produced urease. Glucose was fermented to acetate and lactate. Strain MRM 3/1(T) showed a peptidoglycan type unique among members of the genus Bifidobacterium. The DNA base composition was 64.7 mol% G+C. Almost-complete 16S rRNA, hsp60, clpC and rpoB gene sequences were obtained and phylogenetic relationships were determined. Comparative analysis of 16S rRNA gene sequences showed that strains MRM 3/1(T) and MRM 4/2 had the highest similarities to Bifidobacterium scardovii DSM 13734(T) (94.6%) and Bifidobacterium stellenboschense DSM 23968(T) (94.5%). Analysis of hsp60 showed that both strains were closely related to B. stellenboschense DSM 23968(T) (97.5% similarity); however, despite this high degree of similarity, our isolates could be distinguished from B. stellenboschense DSM 23968(T) by low levels of DNA-DNA relatedness (30.4% with MRM 3/1(T)). Strains MRM 3/1(T) and MRM 4/2 were located in an actinobacterial cluster and were more closely related to the genus Bifidobacterium than to other genera in the family Bifidobacteriaceae. On the basis of these results, strains MRM 3/1(T) and MRM 4/2 represent a novel species within the genus Bifidobacterium, for which the name Bifidobacterium aesculapii sp. nov. is proposed; the type strain is MRM 3/1(T) ( = DSM 26737(T) = JCM 18761(T)).
|
| 巻・号 |
64(Pt 8)
|
| ページ |
2819-2827
|
| 公開日 |
2014-8-1
|
| DOI |
10.1099/ijs.0.056937-0
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| PMID |
24867172
|
| MeSH |
Aldehyde-Lyases / chemistry
Animals
Bacterial Typing Techniques
Base Composition
Bifidobacterium / classification*
Bifidobacterium / genetics
Bifidobacterium / isolation & purification
Callithrix / microbiology*
DNA, Bacterial / genetics
Feces / microbiology*
Genes, Bacterial
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| IF |
2.415
|
| 引用数 |
21
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 18761
JCM 18762 |
