RRC ID |
35121
|
著者 |
Komiya R, Ohyanagi H, Niihama M, Watanabe T, Nakano M, Kurata N, Nonomura K.
|
タイトル |
Rice germline-specific Argonaute MEL1 protein binds to phasiRNAs generated from more than 700 lincRNAs.
|
ジャーナル |
Plant J
|
Abstract |
Small RNAs that interact with Argonaute (AGO) proteins play central roles in RNA-mediated silencing. MEIOSIS ARRESTED AT LEPTOTENE1 (MEL1), a rice AGO, has specific functions in the development of pre-meiotic germ cells and the progression of meiosis. Here, we show that MEL1, which is located mostly in the cytoplasm of germ cells, associates preferentially with 21-nucleotide phased small interfering RNAs (phasiRNAs) that bear a 5'-terminal cytosine. Most phasiRNAs are derived from 1171 intergenic clusters distributed on all rice chromosomes. From these clusters, over 700 large intergenic, non-coding RNAs (lincRNAs) that contain the consensus sequence complementary to miR2118 are transcribed specifically in inflorescences, and cleaved within the miR2118 site. Cleaved lincRNAs are processed via DICER-LIKE4 (DCL4) protein, resulting in production of phasiRNAs. This study provides the evidence that the miR2118-dependent and the DCL4-dependent pathways are both required for biogenesis of 21-nt phasiRNAs associated with germline-specific MEL1 AGO in rice, and over 700 lincRNAs are key factors for induction of this biogenesis during reproductive-specific stages.
|
巻・号 |
78(3)
|
ページ |
385-97
|
公開日 |
2014-5-1
|
DOI |
10.1111/tpj.12483
|
PMID |
24635777
|
MeSH |
Argonaute Proteins / genetics
Argonaute Proteins / metabolism*
Chromosomes, Plant
Gene Expression Regulation, Plant
Meiosis
Oryza / genetics*
Plant Proteins / genetics
Plant Proteins / metabolism*
RNA, Long Noncoding*
RNA, Plant / genetics
RNA, Plant / metabolism
RNA, Small Interfering / metabolism*
|
IF |
6.141
|
引用数 |
92
|
WOS 分野
|
PLANT SCIENCES
|
リソース情報 |
イネ |
Sterile strain (STER-NIG0009) (MEL1) |