論文 - 詳細
| RRC ID | 35301 |
|---|---|
| 著者 | Krin E, Danchin A, Soutourina O. |
| タイトル | RcsB plays a central role in H-NS-dependent regulation of motility and acid stress resistance in Escherichia coli. |
| ジャーナル | Res Microbiol |
| Abstract |
In Escherichia coli, hns mutants lack flagellar motility and display an increase in acid stress resistance. Spontaneous phenotypic revertants showed reversion of both H-NS-controlled phenotypes. In the present study, suppressor mutations were identified in the rcsB gene. In addition to RcsA, our experiments establish that H-NS indirectly controlled the RcsB regulator via repression of RcsD. We also show that RcsB(D56E), mimicking phosphorylated RcsB, interacts with GadE to form a RcsB-P/GadE complex, a general direct regulator of glutamate-, arginine- and lysine-dependent acid resistance pathways. In addition, we showed that H-NS positively affects motility via the flhDC master operon repression by RcsB. This substantiates the central role of RcsB in H-NS-mediated control of motility and acid stress resistance. |
| 巻・号 | 161(5) |
| ページ | 363-71 |
| 公開日 | 2010-6-1 |
| DOI | 10.1016/j.resmic.2010.04.002 |
| PII | S0923-2508(10)00084-7 |
| PMID | 20435136 |
| MeSH | Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli / physiology* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Fimbriae Proteins / genetics Fimbriae Proteins / metabolism* Flagella / genetics* Flagella / metabolism Gene Expression Regulation, Bacterial* Hydrogen-Ion Concentration Molecular Sequence Data Movement Operon Phosphorylation Phosphotransferases / genetics Phosphotransferases / metabolism* Regulatory Elements, Transcriptional Stress, Physiological* Suppression, Genetic Trans-Activators / genetics Trans-Activators / metabolism Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 3.217 |
| 引用数 | 37 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | NA |